gms | German Medical Science

76th Annual Meeting of the German Society of Oto-Rhino-Laryngology, Head and Neck Surgery

German Society of Oto-Rhino-Laryngology, Head and Neck Surgery

04.05. - 08.05.2005, Erfurt

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Identification of deregulated functional groups on mRNA-level by microarray-technique in head and neck carcinomas

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  • corresponding author Christian Cordes - Department of Otorhinolaryngology, Head and Neck Surgery, University Hospital Schleswig-Holstein, Campus Kiel, Germany
  • Stefan Gottschlich - Department of Otorhinolaryngology, Head and Neck Surgery, University Hospital Schleswig-Holstein, Campus Kiel, Germany
  • Robert Häsler - I. Department of Internal Medicine, University Hospital Schleswig-Holstein, Campus Kiel, Germany
  • Petra Ambrosch - Department of Otorhinolaryngology, Head and Neck Surgery, University Hospital Schleswig-Holstein, Campus Kiel, Germany

Deutsche Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie. 76. Jahresversammlung der Deutschen Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie e.V.. Erfurt, 04.-08.05.2005. Düsseldorf, Köln: German Medical Science; 2005. Doc05hno462

The electronic version of this article is the complete one and can be found online at: http://www.egms.de/en/meetings/hno2005/05hno151.shtml

Published: September 22, 2005

© 2005 Cordes et al.
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Outline

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The exact molecular mechanismns coursing the development of squamous cell carcinomas in head and neck region are mostly unknown. Identification of genetic changes which are involved in carcinogenesis might play a key role for improvement of diagnosis, therapy and finally prognosis of patients with carcinomas in head and neck region. One method to identify those mechanismns is the so called microarray-technique. It allows to measure gene expression of hundreds of genes of one cell or of one tissue simultaneously. We measured the expression of 408 apoptosis associated genes by microarray-technique in normal upper aerodigestive tract mucosa (n=3), in cancer edge (n=2) and in cancer center (n=4) of squamous cell carcinomas of the upper aero-digestive-tract. For statistical analysis a two dimensional hierarchical clustering with statistical predefined groups was performed. 11 with antiapoptotical processes connected genes were identified which were upregulated. 15 with proapoptotic associated genes as well as 5 with DNA-replication and chromosome cycle associated genes were downregulated in tumor tissue. Furthermore 6 of 8 with lymphocyte activation associated genes were downregulated in tumor tissue. These results allowed a classification of tissue in the 3 predefined groups by 22 marker genes. These results are a first step in diagnostic usage of microarrays in squamous cell carcinomas of head and neck region and might improve the understanding of mechanismns of carcinogenesis in head and neck region.