gms | German Medical Science

76. Jahresversammlung der Deutschen Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie e. V.

Deutsche Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie e. V.

04.05. - 08.05.2005, Erfurt

Identification of deregulated functional groups on mRNA-level by microarray-technique in head and neck carcinomas

Meeting Abstract

  • corresponding author Christian Cordes - Department of Otorhinolaryngology, Head and Neck Surgery, University Hospital Schleswig-Holstein, Campus Kiel, Germany
  • Stefan Gottschlich - Department of Otorhinolaryngology, Head and Neck Surgery, University Hospital Schleswig-Holstein, Campus Kiel, Germany
  • Robert Häsler - I. Department of Internal Medicine, University Hospital Schleswig-Holstein, Campus Kiel, Germany
  • Petra Ambrosch - Department of Otorhinolaryngology, Head and Neck Surgery, University Hospital Schleswig-Holstein, Campus Kiel, Germany

Deutsche Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie. 76. Jahresversammlung der Deutschen Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie e.V.. Erfurt, 04.-08.05.2005. Düsseldorf, Köln: German Medical Science; 2005. Doc05hno462

Die elektronische Version dieses Artikels ist vollständig und ist verfügbar unter: http://www.egms.de/de/meetings/hno2005/05hno151.shtml

Veröffentlicht: 22. September 2005

© 2005 Cordes et al.
Dieser Artikel ist ein Open Access-Artikel und steht unter den Creative Commons Lizenzbedingungen (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.de). Er darf vervielf&aauml;ltigt, verbreitet und &oauml;ffentlich zug&aauml;nglich gemacht werden, vorausgesetzt dass Autor und Quelle genannt werden.


Gliederung

Text

The exact molecular mechanismns coursing the development of squamous cell carcinomas in head and neck region are mostly unknown. Identification of genetic changes which are involved in carcinogenesis might play a key role for improvement of diagnosis, therapy and finally prognosis of patients with carcinomas in head and neck region. One method to identify those mechanismns is the so called microarray-technique. It allows to measure gene expression of hundreds of genes of one cell or of one tissue simultaneously. We measured the expression of 408 apoptosis associated genes by microarray-technique in normal upper aerodigestive tract mucosa (n=3), in cancer edge (n=2) and in cancer center (n=4) of squamous cell carcinomas of the upper aero-digestive-tract. For statistical analysis a two dimensional hierarchical clustering with statistical predefined groups was performed. 11 with antiapoptotical processes connected genes were identified which were upregulated. 15 with proapoptotic associated genes as well as 5 with DNA-replication and chromosome cycle associated genes were downregulated in tumor tissue. Furthermore 6 of 8 with lymphocyte activation associated genes were downregulated in tumor tissue. These results allowed a classification of tissue in the 3 predefined groups by 22 marker genes. These results are a first step in diagnostic usage of microarrays in squamous cell carcinomas of head and neck region and might improve the understanding of mechanismns of carcinogenesis in head and neck region.