Article
Up-regulation of beta-catenin in external auditory canal cholesteatoma
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Published: | September 22, 2005 |
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The external auditory canal cholesteatoma (EACC) is a rare disease with hyperprolieration and destructive growth in the adjacent structures. Down-regulation of beta-catenin is a pivotal factor for loose tissue integrity and invasiveness.. Transforming growth factor beta 1 (TGF-beta1) was reported to decrease beta-catenin in mammary epithelium. We investigated the abrogation of TGF-beta1 and beta-catenin expression in EACC culture cells. Cultured EACC-specimens were incubated with 6 micromol TGF-beta1 antisense. After 48h, expression of beta-catenin was determined by means of immunohistochemistry. The cells showed an increased mural reactivity to beta-catenin, and intracellular reactivity was unchanged. The predominant membranous location after treatment with TGF-beta1 antisense suggests increased tendency of the cells for tissue formation and strong cell-cell adhesion rather than migratory and invasive character, and thus TGF-beta1 antisense application is a useful therapeutical strategy.