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68. Jahrestagung der Deutschen Gesellschaft für Unfallchirurgie
90. Tagung der Deutschen Gesellschaft für Orthopädie und Orthopädische Chirurgie
45. Tagung des Berufsverbandes der Fachärzte für Orthopädie in Zusammenarbeit mit dem Deutschen Verband für Physiotherapie – Zentralverband der Physiotherapeuten/Krankengymnasten

19. bis 23.10.2004, Berlin

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Comparison of hMSC vitality and osteogenic potential on different metallic implant surfaces

Meeting Abstract (DGOOC 2004)

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  • presenting/speaker W. Caro - Universität Regensburg, Orthopädie, Bad Abbach
  • T. Kalteis - Universität Regensburg, Orthopädie, Bad Abbach
  • J. Schaumburger - Universität Regensburg, Orthopädie, Bad Abbach
  • O. Wiech - Universität Regensburg, Orthopädie, Bad Abbach
  • S. Grässel - Universität Regensburg, Orthopädie, Bad Abbach
  • J. Grifka - Universität Regensburg, Orthopädie, Bad Abbach

Deutsche Gesellschaft für Unfallchirurgie. Deutsche Gesellschaft für Orthopädie und orthopädische Chirurgie. Berufsverband der Fachärzte für Orthopädie. 68. Jahrestagung der Deutschen Gesellschaft für Unfallchirurgie, 90. Tagung der Deutschen Gesellschaft für Orthopädie und Orthopädische Chirurgie und 45. Tagung des Berufsverbandes der Fachärzte für Orthopädie. Berlin, 19.-23.10.2004. Düsseldorf, Köln: German Medical Science; 2004. Doc04dguN9-1540

The electronic version of this article is the complete one and can be found online at: http://www.egms.de/en/meetings/dgu2004/04dgu0717.shtml

Published: October 19, 2004

© 2004 Caro et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.en). You are free: to Share – to copy, distribute and transmit the work, provided the original author and source are credited.

Outline

Text

Introduction

In Orthopedic surgery revisions of total joint replacements in case of poor bone stock conditions are still challenging. To improve osseointegration of metallic implants we established an in vitro model to grow hMSCs on two different metallic surfaces common in TJR.

Methods

hMSCs from 8 allogenic donors of various ages were expanded on titanium alloys employing two different surface conditions. Polystyrene was used as a control. As indicative parameters for biocompatibility primary cell number, cell proliferation, and cell vitality (WST-1, BrdU, LDH) were described on days 4, 8, 12 and 16. Differentiation was initiated by adding dexamethasone, ascorbic acid, and ß-glycerophosphate as recommended. After induction of osteogenic differentiation cell vitality and alkaline phosphatase actvity were tested routinely. After 16 days hMSCs and bonelike cells were pictured via scanning electron microscopy (SEM) and via light microscopy.

Results

We observed a increase in proliferation and vitality rates until day 12 on all surfaces. Afterwards both parameters seemed to slow down. Cells seeded on plasmapore coated titanium alloy performed always best. The induction medium had no influence on vitality. Alkaline phosphatase activity increased dramatically after 16 days. SEM/lightmicroscopy showed the presence of hMSCs and bonelike cells on all tested surfaces.

Conclusions

Our investigations indicated that both metallic surfaces are non-toxic, however, plasmapore coated titanium alloy has a much better vitality performance than plain titanium alloy. Both have no effect on hMSC differentitation into bonelike cells.