Artikel
Comparison of hMSC vitality and osteogenic potential on different metallic implant surfaces
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Autoren
Veröffentlicht: | 19. Oktober 2004 |
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Gliederung
Text
Introduction
In Orthopedic surgery revisions of total joint replacements in case of poor bone stock conditions are still challenging. To improve osseointegration of metallic implants we established an in vitro model to grow hMSCs on two different metallic surfaces common in TJR.
Methods
hMSCs from 8 allogenic donors of various ages were expanded on titanium alloys employing two different surface conditions. Polystyrene was used as a control. As indicative parameters for biocompatibility primary cell number, cell proliferation, and cell vitality (WST-1, BrdU, LDH) were described on days 4, 8, 12 and 16. Differentiation was initiated by adding dexamethasone, ascorbic acid, and ß-glycerophosphate as recommended. After induction of osteogenic differentiation cell vitality and alkaline phosphatase actvity were tested routinely. After 16 days hMSCs and bonelike cells were pictured via scanning electron microscopy (SEM) and via light microscopy.
Results
We observed a increase in proliferation and vitality rates until day 12 on all surfaces. Afterwards both parameters seemed to slow down. Cells seeded on plasmapore coated titanium alloy performed always best. The induction medium had no influence on vitality. Alkaline phosphatase activity increased dramatically after 16 days. SEM/lightmicroscopy showed the presence of hMSCs and bonelike cells on all tested surfaces.
Conclusions
Our investigations indicated that both metallic surfaces are non-toxic, however, plasmapore coated titanium alloy has a much better vitality performance than plain titanium alloy. Both have no effect on hMSC differentitation into bonelike cells.