Article
The immunohistochemical expression of calcitonin receptor-like receptor (CRLR) in human gliomas
Die immunhistochemische Expression des Calcitonin Receptor-like Rezeptors (CRLR) in humanen Gliomen
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Published: | May 4, 2005 |
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Outline
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Objective
Gliomas are the most common primary tumours of the central nervous system and their rapid growth is associated and dependent on neovascularisation. The influence of different factors promoting tumor growth have been shown, previously. One of these is Adrenomedullin. There is evidence that the tumorigenic actions of adrenomedullin are promoted by a receptor named calcitonin receptor-like receptor (CRLR). The aim of our study was to find out whether CRLR protein is expressed in human gliomas, to determine its cellular distribution and to figure out possible correlations to the tumor grade.
Methods
RNA of three human glioma cell-lines was extracted, transscripted into cDNA and amplified by PCR. The PCR-products were verified by polyacrylamide gel electrophoresis. Immunohis-tochemistry was performed on 95 sections of tumor biopsies. The Peroxidase-antiperoxidase method was used for visualisation of the antigens. An affinity-purified antibody, previously raised in rabbit against commercially synthesised human CRLR-sequence, was applied. The specimens were analysed for CRLR immunostaining and directly compared with the staining pattern of glial fibrillary acid protein (GFAP).
Results
All analysed glioma cell-lines were positive for CRLR-mRNA. In human gliomas all tumor specimens were positive for CRLR-immunostaining. There was an increasing CRLR-immunoreactivity up to grade III gliomas, but a decline of immunoreactivity in glioblastomas. CRLR immunostaining was predominantly positive in vascular endothelial cells and astrocytic tumor cells. In contrast to the endothelium of blood vessels which were constantly CRLR immunostained, tumour cells were stained inconstantly.
Conclusions
The study has shown for the first time that CRLR protein is present in human glioma tissue. The expression of the receptor in endothelial and astrocytic tumor cells is consistent with the fact that its endogenous ligand, adrenomedullin, is possibly involved in angiogenesis and carcinogenic tumor progression of gliomas.