gms | German Medical Science

56. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie e. V. (DGNC)
3èmes journées françaises de Neurochirurgie (SFNC)

Deutsche Gesellschaft für Neurochirurgie e. V.
Société Française de Neurochirurgie

07. bis 11.05.2005, Strasbourg

The immunohistochemical expression of calcitonin receptor-like receptor (CRLR) in human gliomas

Die immunhistochemische Expression des Calcitonin Receptor-like Rezeptors (CRLR) in humanen Gliomen

Meeting Abstract

  • corresponding author C. Kappus - Klinik für Neurochirurgie der Philipps-Universität Marburg
  • L. Benes - Klinik für Neurochirurgie der Philipps-Universität Marburg
  • H. Bertalanffy - Klinik für Neurochirurgie der Philipps-Universität Marburg
  • H. D. Mennel - Abteilung für Neuropathologie der Philipps-Universität Marburg
  • S. Hagner - Institut für Normale und Pathologische Physiologie der Philipps-Universität Marburg

Deutsche Gesellschaft für Neurochirurgie. Société Française de Neurochirurgie. 56. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie e.V. (DGNC), 3èmes journées françaises de Neurochirurgie (SFNC). Strasbourg, 07.-11.05.2005. Düsseldorf, Köln: German Medical Science; 2005. DocP173

The electronic version of this article is the complete one and can be found online at:

Published: May 4, 2005

© 2005 Kappus et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( You are free: to Share – to copy, distribute and transmit the work, provided the original author and source are credited.




Gliomas are the most common primary tumours of the central nervous system and their rapid growth is associated and dependent on neovascularisation. The influence of different factors promoting tumor growth have been shown, previously. One of these is Adrenomedullin. There is evidence that the tumorigenic actions of adrenomedullin are promoted by a receptor named calcitonin receptor-like receptor (CRLR). The aim of our study was to find out whether CRLR protein is expressed in human gliomas, to determine its cellular distribution and to figure out possible correlations to the tumor grade.


RNA of three human glioma cell-lines was extracted, transscripted into cDNA and amplified by PCR. The PCR-products were verified by polyacrylamide gel electrophoresis. Immunohis-tochemistry was performed on 95 sections of tumor biopsies. The Peroxidase-antiperoxidase method was used for visualisation of the antigens. An affinity-purified antibody, previously raised in rabbit against commercially synthesised human CRLR-sequence, was applied. The specimens were analysed for CRLR immunostaining and directly compared with the staining pattern of glial fibrillary acid protein (GFAP).


All analysed glioma cell-lines were positive for CRLR-mRNA. In human gliomas all tumor specimens were positive for CRLR-immunostaining. There was an increasing CRLR-immunoreactivity up to grade III gliomas, but a decline of immunoreactivity in glioblastomas. CRLR immunostaining was predominantly positive in vascular endothelial cells and astrocytic tumor cells. In contrast to the endothelium of blood vessels which were constantly CRLR immunostained, tumour cells were stained inconstantly.


The study has shown for the first time that CRLR protein is present in human glioma tissue. The expression of the receptor in endothelial and astrocytic tumor cells is consistent with the fact that its endogenous ligand, adrenomedullin, is possibly involved in angiogenesis and carcinogenic tumor progression of gliomas.