Artikel
Solar elastosis, p53 mutation and human papillom virus in ocular surface squamous neoplasia
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Autoren
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R. Guthoff
- Department of Ophthalmology, Julius Maximilians University Würzburg, Würzburg
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D. Krahl
- Institute of Dermatohistology, Heidelberg
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C. Rose
- Department of Dermatology, Julius Maximilians University Würzburg, Würzburg
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T. Rüdiger
- Institute of Pathology, Julius Maximilians University Würzburg, Würzburg
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A. Marx
- Institute of Pathology, Julius Maximilians University Würzburg, Würzburg
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P. Ströbel
- Institute of Pathology, Julius Maximilians University Würzburg, Würzburg;
| Veröffentlicht: | 22. September 2004 |
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Gliederung
Text
Objective
UV radiation and human papillomavirus have been implicated in the development of ocular surface squamous neoplasia (OSSN). We analyzed the clinical and histological appearance and the role of human papillomavirus (HPV) infection and p53 mutation in OSSN.
Methods
31 formalin fixed and paraffin embedded specimens with OSSN (7 conjunctival intraepithelial neoplasias (CIN), 17 carcinomas in situ (Cis) and 7 invasive squamous cell carcinomas (SCC)) and 3 disease free conjunctiva samples were studied by haematoxylin and eosin stains for evaluation of solar elastosis and immunohistochemically to assess the expression of HPV, ki-67, p53, p21, Caspase 3 and bcl-2. For classification of CIN, ki-67 was used as a marker analogous to the grading of cervical carcinoma.
Results
Solar elastosis was present in 5 CIN (71%), in 15 of 17 Cis (88%) and 5 SCC (71%) and none of the 5 normal conjunctiva. HPV was identified in 1 CIN (14%), in 3 Cis (18%) and in 1 SCC (18%), and all showed solar elastosis. p53 mutation was detected in 2 SCC (29%), in 2 Cis (12%) and in 1 CIN (14%). Four out of 5 HPV positive OSSN revealed a papillomatous and 1 a leukoplakic clinical appearance. None of the HPV postive OSSN showed a cystic-gelatinous morphology.
Conclusions
For the highly differenciated OSSN it seems likely that deleting p53 mutation may occur in advanced stages due to limited apoptosis capability. There may be a correlation between HPV infection and papillomatous rather than cystic-gelatinous appearance of OSSN. Solar elastosis due to UV radiation damage was frequently present in OSSN.
