gms | German Medical Science

102. Jahrestagung der DOG

Deutsche Ophthalmologische Gesellschaft e. V.

23. bis 26.09.2004, Berlin

Solar elastosis, p53 mutation and human papillom virus in ocular surface squamous neoplasia

Meeting Abstract

  • corresponding author R. Guthoff - Department of Ophthalmology, Julius Maximilians University Würzburg, Würzburg
  • D. Krahl - Institute of Dermatohistology, Heidelberg
  • C. Rose - Department of Dermatology, Julius Maximilians University Würzburg, Würzburg
  • T. Rüdiger - Institute of Pathology, Julius Maximilians University Würzburg, Würzburg
  • A. Marx - Institute of Pathology, Julius Maximilians University Würzburg, Würzburg
  • P. Ströbel - Institute of Pathology, Julius Maximilians University Würzburg, Würzburg;

Evidenzbasierte Medizin - Anspruch und Wirklichkeit. 102. Jahrestagung der Deutschen Ophthalmologischen Gesellschaft. Berlin, 23.-26.09.2004. Düsseldorf, Köln: German Medical Science; 2004. Doc04dogDO.07.06

The electronic version of this article is the complete one and can be found online at:

Published: September 22, 2004

© 2004 Guthoff et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( You are free: to Share – to copy, distribute and transmit the work, provided the original author and source are credited.




UV radiation and human papillomavirus have been implicated in the development of ocular surface squamous neoplasia (OSSN). We analyzed the clinical and histological appearance and the role of human papillomavirus (HPV) infection and p53 mutation in OSSN.


31 formalin fixed and paraffin embedded specimens with OSSN (7 conjunctival intraepithelial neoplasias (CIN), 17 carcinomas in situ (Cis) and 7 invasive squamous cell carcinomas (SCC)) and 3 disease free conjunctiva samples were studied by haematoxylin and eosin stains for evaluation of solar elastosis and immunohistochemically to assess the expression of HPV, ki-67, p53, p21, Caspase 3 and bcl-2. For classification of CIN, ki-67 was used as a marker analogous to the grading of cervical carcinoma.


Solar elastosis was present in 5 CIN (71%), in 15 of 17 Cis (88%) and 5 SCC (71%) and none of the 5 normal conjunctiva. HPV was identified in 1 CIN (14%), in 3 Cis (18%) and in 1 SCC (18%), and all showed solar elastosis. p53 mutation was detected in 2 SCC (29%), in 2 Cis (12%) and in 1 CIN (14%). Four out of 5 HPV positive OSSN revealed a papillomatous and 1 a leukoplakic clinical appearance. None of the HPV postive OSSN showed a cystic-gelatinous morphology.


For the highly differenciated OSSN it seems likely that deleting p53 mutation may occur in advanced stages due to limited apoptosis capability. There may be a correlation between HPV infection and papillomatous rather than cystic-gelatinous appearance of OSSN. Solar elastosis due to UV radiation damage was frequently present in OSSN.