Artikel
Expression of estrogen receptor ß- isoforms in invasive breast cancer cell lines
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Autoren
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Nicole Petzke
- Department of Obstetrics and Gynecology, University of Tübingen, Tuebingen, Deutschland
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Harald Seeger
- Section of Endocrinology and Menopause, University of Tübingen
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Martin Wurster
- Department of Obstetrics and Gynecology, University of Tübingen
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Tanja Fehm
- Department of Obstetrics and Gynecology, University of Tübingen
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Christina Schuetz
- Department of Obstetrics and Gynecology, University of Tübingen
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Erich Solomayer
- Department of Obstetrics and Gynecology, University of Tübingen
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Diethelm Wallwiener
- Department of Obstetrics and Gynecology, University of Tübingen
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Hans Neubauer
- Department of Obstetrics and Gynecology, University of Tübingen
| Veröffentlicht: | 20. März 2006 |
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Gliederung
Text
Estrogen receptor (ER) ß is involved in physiological and pathological processes in the mammary gland. Different ERß-isoforms have been identified in the past, without providing their exact function. In order to determine the biological function of distinct ERß-isoforms it is important to get detailed information about their expressional regulation. For this purpose we have established four classic multiplex- and six real-time-PCRs to quantify wild-type ERß (ERßwt), ERßcx-, ERßD5-, ERß5-splice variants. Expression profiling of ERß-splice variants in four standard-cultured breast cancer cell lines (Tamoxifen-resistant MCF7 (MCF7Tam res) cell line, its parental MCF7 cell line, MCF10A and MDA-MB 231 cells) reveal that ERßwt is expressed in MCF7Tam res and MDA-MB 231 cells. Expression of ERßwt cannot be detected in MCF7 cells. On the other hand MCF7 cells express ERß5 which could not be detected in MCF7Tam res. Taken together, the expression patterns of ERß-isoforms in MDA-MB 231- and MCF7Tam res-cells are more similar than in MCF7- and MCF7Tam res-cells. As MDA-MB 231 cells are invasive, these data suggest that expression pattern of ERß isoforms might be correlated with invasive capacity. Invasion assays show that MCF-7Tam res-cells, like MDA-MB 231-cells, are indeed highly invasive compared to parental MCF7 cells. Additionally, ERß expression profiling of invasive and non-invasive subfractions of MDA-MB 231 cells reveals differential expression of ERß-isoforms. In summary, our data indicate that differential expression of ERß-isoforms might be correlated with invasiveness of breast tumor cells. An extended investigation with more cell lines is under work.
