gms | German Medical Science

27th German Cancer Congress Berlin 2006

German Cancer Society (Frankfurt/M.)

22. - 26.03.2006, Berlin

Expression of estrogen receptor ▀- isoforms in invasive breast cancer cell lines

Meeting Abstract

  • corresponding author presenting/speaker Nicole Petzke - Department of Obstetrics and Gynecology, University of TŘbingen, Tuebingen, Deutschland
  • Harald Seeger - Section of Endocrinology and Menopause, University of TŘbingen
  • Martin Wurster - Department of Obstetrics and Gynecology, University of TŘbingen
  • Tanja Fehm - Department of Obstetrics and Gynecology, University of TŘbingen
  • Christina Schuetz - Department of Obstetrics and Gynecology, University of TŘbingen
  • Erich Solomayer - Department of Obstetrics and Gynecology, University of TŘbingen
  • Diethelm Wallwiener - Department of Obstetrics and Gynecology, University of TŘbingen
  • Hans Neubauer - Department of Obstetrics and Gynecology, University of TŘbingen

27. Deutscher Krebskongress. Berlin, 22.-26.03.2006. DŘsseldorf, K÷ln: German Medical Science; 2006. DocPO080

The electronic version of this article is the complete one and can be found online at: http://www.egms.de/en/meetings/dkk2006/06dkk190.shtml

Published: March 20, 2006

© 2006 Petzke et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.en). You are free: to Share – to copy, distribute and transmit the work, provided the original author and source are credited.


Outline

Text

Estrogen receptor (ER) ▀ is involved in physiological and pathological processes in the mammary gland. Different ER▀-isoforms have been identified in the past, without providing their exact function. In order to determine the biological function of distinct ER▀-isoforms it is important to get detailed information about their expressional regulation. For this purpose we have established four classic multiplex- and six real-time-PCRs to quantify wild-type ER▀ (ER▀wt), ER▀cx-, ER▀D5-, ER▀5-splice variants. Expression profiling of ER▀-splice variants in four standard-cultured breast cancer cell lines (Tamoxifen-resistant MCF7 (MCF7Tam res) cell line, its parental MCF7 cell line, MCF10A and MDA-MB 231 cells) reveal that ER▀wt is expressed in MCF7Tam res and MDA-MB 231 cells. Expression of ER▀wt cannot be detected in MCF7 cells. On the other hand MCF7 cells express ER▀5 which could not be detected in MCF7Tam res. Taken together, the expression patterns of ER▀-isoforms in MDA-MB 231- and MCF7Tam res-cells are more similar than in MCF7- and MCF7Tam res-cells. As MDA-MB 231 cells are invasive, these data suggest that expression pattern of ER▀ isoforms might be correlated with invasive capacity. Invasion assays show that MCF-7Tam res-cells, like MDA-MB 231-cells, are indeed highly invasive compared to parental MCF7 cells. Additionally, ER▀ expression profiling of invasive and non-invasive subfractions of MDA-MB 231 cells reveals differential expression of ER▀-isoforms. In summary, our data indicate that differential expression of ER▀-isoforms might be correlated with invasiveness of breast tumor cells. An extended investigation with more cell lines is under work.