gms | German Medical Science

26th Annual Meeting of the German Retina Society

German Retina Society

27.09.2013, Hamburg

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Upregulation of miRNA in a single primary vitreoretinal lymphoma specimen: A new diagnostic approach?

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  • Vinodh Kakkassery - Department of Ophthalmology, Ruhr-Universität Bochum, Germany
  • M Elling - Department of Ophthalmology, Ruhr-Universität Bochum, Germany
  • D.S. Loos - Department of Ophthalmology, Ruhr-Universität Bochum, Germany
  • H.B. Dick - Department of Ophthalmology, Ruhr-Universität Bochum, Germany
  • U. Schlegel - Department of Neurology, Ruhr-Universität Bochum, Germany
  • R. Schroers - Department of Medicine, Hematology, and Oncology, Ruhr-Universität Bochum, Germany
  • A. Baraniskin - Department of Medicine, Hematology, and Oncology, Ruhr-Universität Bochum, Germany

Retinologische Gesellschaft. 26. Jahrestagung der Retinologischen Gesellschaft. Hamburg, 27.-27.09.2013. Düsseldorf: German Medical Science GMS Publishing House; 2013. Doc13rg34

doi: 10.3205/13rg34, urn:nbn:de:0183-13rg342

Published: August 20, 2013

© 2013 Kakkassery et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.en). You are free: to Share – to copy, distribute and transmit the work, provided the original author and source are credited.

Outline

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Background: Primary vitreoretinal lymphoma (PVRL) is a rare, but high malignant intraocular tumor with limited prognosis. Clinical findings of PVRL may simulate uveitis. Difficulties to confirm diagnosis, even after cytological and immunhistochemical analyses of vitreal specimens, are often. MiRNAs in cerebrospinal fluid have been demonstrated as a reliable marker for primary CNS lymphoma. Objective of this analysis was to evalute preliminary miRNA data in a PVRL specimen.

Methods: A single vitreal specimen from a 53 year old female patient with confirmed PVRL (analysed by the Coupland laboratory/Liverpool) was analysed for miRNAs by Real-Time PCR and compared to controll specimens (7 normal and a single uveitis specimen).

Results: At least 16 fold upregulation of three marker miRNAs has been seen in the PVRL specimen in comparison to the control group of 8 specimens. These differences have also been seen between the PVRL and the uveitis specimen.

Discussion: MiRNAs have the potential to be a valuable diagnostic tool for PRVL and especially to differentiate between PVRL and uveitis. Further analysis with a larger number of patients are necessary to increase first evidence.