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86th Annual Meeting of the German Society of Oto-Rhino-Laryngology, Head and Neck Surgery

German Society of Oto-Rhino-Laryngology, Head and Neck Surgery

13.05. - 16.05.2015, Berlin

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Non-thermal atmospheric pressure plasma – a new option in cancer treatment?

Meeting Abstract

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  • Laura L Rethschulte - Univ. HNO-Klinik Greifswald, Greifswald
  • Achim G. Beule - Univ. HNO-Klinik, Greifswald
  • Katrin Darm - Univ. HNO-Klinik, Greifswald
  • Klaus-Dieter Weltmann - INP Greifswald, Greifswald
  • Werner Hosemann - Univ. HNO-Klinik, Greifswald
  • corresponding author Christian Scharf - Univ. HNO-Klinik, Greifswald

Deutsche Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie. 86. Jahresversammlung der Deutschen Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie. Berlin, 13.-16.05.2015. Düsseldorf: German Medical Science GMS Publishing House; 2015. Doc15hnod251

doi: 10.3205/15hnod251, urn:nbn:de:0183-15hnod2514

Published: March 26, 2015

© 2015 Rethschulte et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License. You are free: to Share - to copy, distribute and transmit the work, provided the original author and source are credited. See license information at http://creativecommons.org/licenses/by-nc-nd/3.0/.

Outline

Text

Atmospheric pressure plasma (APP) is an ionized gas composed of electrons, charged particles, radicals, various excited molecules, UV photons and transient electric fields. Significantly increased levels of reactive oxygen species (ROS) generated by APP treatment can damage DNA, proteins and lipids and can result in apoptosis. Based on the of both, enhancing and inhibiting cellular activity by direct tissue interaction as well as the unique composition of APP, a new minimal-invasive surgical approach of selective cancer cell treatment was suggested. Tumor cells are more sensitive against APP treatment than surrounding epithelial and fibroblast cells.

The aim of the present study was to identify the effects of varying doses of APP treatment on molecular pathways in several tumor cell lines in comparison to airway epithelial and fibroblast cells at several time points after plasma treatment.

Dose dependent survival of APP treated cells analysed by label-free viability determination and cell sizing. A two-dimensional-difference gel-electrophoresis (2D-DIGE) approach was used to quantify the proteomic changes and to evaluate the effects due to APP application. Differentially expressed proteins have been identified by MALDI-TOF/TOF-mass spectrometry. APP-mediated post translational protein modifications have been quantified by a novel Saturn-2D Redox assay based on Cys-interacting compounds. Protein candidates correlating with higher apoptosis ratios in tumor cells have been identified by ingenuity pathway analysis and will be discussed.

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