gms | German Medical Science

Atmospheric pressure plasma (APP) is an ionized gas composed of electrons, charged particles, radicals, various excited molecules, UV photons and transient electric fields. Significantly increased levels of reactive oxygen species (ROS) generated by APP treatment can damage DNA, proteins and lipids and can result in apoptosis. Based on the of both, enhancing and inhibiting cellular activity by direct tissue interaction as well as the unique composition of APP, a new minimal-invasive surgical approach of selective cancer cell treatment was suggested. Tumor cells are more sensitive against APP treatment than surrounding epithelial and fibroblast cells.

The aim of the present study was to identify the effects of varying doses of APP treatment on molecular pathways in several tumor cell lines in comparison to airway epithelial and fibroblast cells at several time points after plasma treatment.

Dose dependent survival of APP treated cells analysed by label-free viability determination and cell sizing. A two-dimensional-difference gel-electrophoresis (2D-DIGE) approach was used to quantify the proteomic changes and to evaluate the effects due to APP application. Differentially expressed proteins have been identified by MALDI-TOF/TOF-mass spectrometry. APP-mediated post translational protein modifications have been quantified by a novel Saturn-2D Redox assay based on Cys-interacting compounds. Protein candidates correlating with higher apoptosis ratios in tumor cells have been identified by ingenuity pathway analysis and will be discussed.

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