gms | German Medical Science

85th Annual Meeting of the German Society of Oto-Rhino-Laryngology, Head and Neck Surgery

German Society of Oto-Rhino-Laryngology, Head and Neck Surgery

28.05. - 01.06.2014, Dortmund

Article

Send article

Evaluation of intra-cochlear structures’ visibility in micro-CT scanning and micro-grinding procedures

Meeting Abstract

Search Medline for

  • corresponding author Marjan Mirsalehi - Hannover medical school, Hannover, Germany
  • Thomas S. Rau - Hannover medical school, institute for Audio-neurotechnology, Hannover
  • Lenka Prielozny - Hannover medical school, institute for Audio-neurotechnology, Hannover
  • Thomas Lenarz - Hannover medical school, institute for Audio-neurotechnology, Hannover
  • Omid Majdani - Hannover medical school, institute for Audio-neurotechnology, Hannover

Deutsche Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie. 85. Jahresversammlung der Deutschen Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie. Dortmund, 28.05.-01.06.2014. Düsseldorf: German Medical Science GMS Publishing House; 2014. Doc14hnod428

doi: 10.3205/14hnod428, urn:nbn:de:0183-14hnod4287

Published: April 14, 2014

© 2014 Mirsalehi et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.en). You are free: to Share – to copy, distribute and transmit the work, provided the original author and source are credited.

Outline

Text

Introduction: Preservation of intra-cochlear anatomy is mandatory in hearing preservation cochlear implantation. Evaluation of histological structures of the cochlea by serial micro-grinding procedure is a reliable method for visualization of intra-cochlear structures, with preserved spatial relations. Another method that appears to be suited for in vitro visualization of inner ear structures is µCT.

In this study, we evaluated the visibility of intra-cochlear structures with these two methods.

Methods: Five human temporal bone specimens were utilized. The specimens were fixed via embedding in epoxy resin. The samples were scanned using micro-CT. Afterwards each sample underwent serial micro-grinding procedure in 100 µm slices, and histological images were stored digitally and reconstructed to a 3D stack.

In both image series we examined the visibility of the microstructure of the cochlear anatomy.

Results: Bony lamina spiralis was visible in micro-CT scan and micro-grinding images. Basilar membrane and spiral ligament were visible just in histological images of micro-grinding procedure. Reissner’s membrane was visible in some images of micro-grinding procedure only.

Conclusion: Anatomical evaluation of intra-cochlear structures by micro-grinding procedure, can lead to more accurate details rather than results achieved by micro-CT scan. This method can be utilized for determining of the degree of violation to the microstructure of the cochlea in Cochlear Implant insertion studies with different electrodes.

Der Erstautor gibt keinen Interessenkonflikt an.