gms | German Medical Science

85. Jahresversammlung der Deutschen Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie e. V.

Deutsche Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie e. V.

28.05. - 01.06.2014, Dortmund

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Evaluation of intra-cochlear structures’ visibility in micro-CT scanning and micro-grinding procedures

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  • corresponding author Marjan Mirsalehi - Hannover medical school, Hannover, Germany
  • Thomas S. Rau - Hannover medical school, institute for Audio-neurotechnology, Hannover
  • Lenka Prielozny - Hannover medical school, institute for Audio-neurotechnology, Hannover
  • Thomas Lenarz - Hannover medical school, institute for Audio-neurotechnology, Hannover
  • Omid Majdani - Hannover medical school, institute for Audio-neurotechnology, Hannover

Deutsche Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie. 85. Jahresversammlung der Deutschen Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie. Dortmund, 28.05.-01.06.2014. Düsseldorf: German Medical Science GMS Publishing House; 2014. Doc14hnod428

doi: 10.3205/14hnod428, urn:nbn:de:0183-14hnod4287

Veröffentlicht: 14. April 2014

© 2014 Mirsalehi et al.
Dieser Artikel ist ein Open Access-Artikel und steht unter den Creative Commons Lizenzbedingungen (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.de). Er darf vervielfältigt, verbreitet und öffentlich zugänglich gemacht werden, vorausgesetzt dass Autor und Quelle genannt werden.

Gliederung

Text

Introduction: Preservation of intra-cochlear anatomy is mandatory in hearing preservation cochlear implantation. Evaluation of histological structures of the cochlea by serial micro-grinding procedure is a reliable method for visualization of intra-cochlear structures, with preserved spatial relations. Another method that appears to be suited for in vitro visualization of inner ear structures is µCT.

In this study, we evaluated the visibility of intra-cochlear structures with these two methods.

Methods: Five human temporal bone specimens were utilized. The specimens were fixed via embedding in epoxy resin. The samples were scanned using micro-CT. Afterwards each sample underwent serial micro-grinding procedure in 100 µm slices, and histological images were stored digitally and reconstructed to a 3D stack.

In both image series we examined the visibility of the microstructure of the cochlear anatomy.

Results: Bony lamina spiralis was visible in micro-CT scan and micro-grinding images. Basilar membrane and spiral ligament were visible just in histological images of micro-grinding procedure. Reissner’s membrane was visible in some images of micro-grinding procedure only.

Conclusion: Anatomical evaluation of intra-cochlear structures by micro-grinding procedure, can lead to more accurate details rather than results achieved by micro-CT scan. This method can be utilized for determining of the degree of violation to the microstructure of the cochlea in Cochlear Implant insertion studies with different electrodes.

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