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83rd Annual Meeting of the German Society of Oto-Rhino-Laryngology, Head and Neck Surgery

German Society of Oto-Rhino-Laryngology, Head and Neck Surgery

16.05. - 20.05.2012, Mainz

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Salicylate enhance the expression and function of NMDA receptors in cochlear spiral ganglion neurons

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  • corresponding author Jiping Su - Guangxi Medical University, Nanning, P.R. China
  • Meng Gao - Guangxi Medical University, Nanning, P.R. China
  • Xiuyou Fang - Guangxi Medical University, Nanning, P.R. China
  • Shuang Feng - Guangxi Medical University, Nanning, P.R. China
  • Lili Deng - Guangxi Medical University, Nanning, P.R. China

Deutsche Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie. 83. Jahresversammlung der Deutschen Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie. Mainz, 16.-20.05.2012. Düsseldorf: German Medical Science GMS Publishing House; 2012. Doc12hnod546

doi: 10.3205/12hnod546, urn:nbn:de:0183-12hnod5468

Published: April 4, 2012

© 2012 Su et al.
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Outline

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Objective: To explore the effect of salicylate on the expression and function of NMDA receptors in spiral ganglion neurons (SGNs).

Methods: The NR1 subunit mRNA of NMDA receptor in modiolus tissues were detected by Real time fluorescent quantitative PCR. The NMDA receptor whole-cell currents were recorded by using patch clamp in SGNs.

Results: Compared with control group, salicylate significantly increased the mRNA level of NR1 subunit in SGNs. NMDA of concentrations ranging from 0.1 mM to10 mM could evoke without current in SGNs. NMDA (0.1 mM, 0.5 mM) applied with salicylate (5 mM), however, induced inward currents (212.6±15.2pA，n=5；607.9±44.3pA，n=5) with dose-dependent manner, which could be inhibited by APV. Salicylate alone did not produce any current in SGNs.

Conclusion: Salicylate increases the expression of NMDA receptor, and facilitates the current mediated by NMDA receptor in SGNs.