Article
The impact of OCT-4 and VPA on the Hep-2 cell xenografts in nude mice and its mechanism
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Authors
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Yulin Zhao
- The First Affiliated Hospital of Zhengzhou University, Zhengzhou, P.R. China
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Junhui Zhang
- The First Affiliated Hospital of Zhengzhou University, Zhengzhou, P.R. China
| Published: | April 4, 2012 |
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Outline
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Objective： To further explore the laryngeal cancer occurrence and development mechanism and to find inhibition methods for laryngeal cancer occurrence and development and provide a theoretical basis for therapeutic approaches.
Method: After establishment of human laryngeal cancer xenografts in nude mice were divided into the control group, the pcDNA3.1-OCT-4-2 group, the pSUPER- EGFP-OCT-4-c group and the VPA experimental group, Weekly measureed each tumor size and nude body mass, drawn tumor volume growth curves. The mice were killed and removed tumor weighed to calculate tumor inhibition rate at termination of treatment.
Results: The volume of the tumors in pcDNA3.1-OCT-4-2 nude mice group was significantly higher than the control group, the difference between the two groups was statistically significant (P<0.05). The tumor volume in pSUPER-EGFP-OCT-4-c group and the VPA group were significantly smaller than the tumor volume in control group, the difference among the three groups were statistically significant (P <0.05).
Conclusion: The expression of OCT-4 protein were higher in laryngeal cancer tissues. The expression of OCT-4 had significant relations with histological grade and lymphatic metastasis，and have significantly positive correlations with the expression of Survivin in laryngeal cancer tissues. The OCT-4 gene can promote the Hep-2 cell proliferation activity and cloning capability, while inhibiting the OCT-4 expression will lead to cell proliferation activity and cloning capability inhibition, block cells in the G0/G1 phase and induce apoptosis，its mechanism were related to inhibition of p-STAT3 and Survivin expression.
