gms | German Medical Science

83rd Annual Meeting of the German Society of Oto-Rhino-Laryngology, Head and Neck Surgery

German Society of Oto-Rhino-Laryngology, Head and Neck Surgery

16.05. - 20.05.2012, Mainz

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Identification of genes associated with laryngeal squamous cell carcinoma by cDNA microarray

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  • corresponding author Xiaohui Shen - Dept. of ORL, HNS, Drum Tower Hospital, Nanjing, China
  • Ni Rongshen - Dept. of ORL, HNS, BenQ Medical Center, Nanjing, China

Deutsche Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie. 83. Jahresversammlung der Deutschen Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie. Mainz, 16.-20.05.2012. Düsseldorf: German Medical Science GMS Publishing House; 2012. Doc12hnod309

doi: 10.3205/12hnod309, urn:nbn:de:0183-12hnod3092

Published: April 4, 2012

© 2012 Shen et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.en). You are free: to Share – to copy, distribute and transmit the work, provided the original author and source are credited.

Outline

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Objective: In order to identify genes involved in tumorigenesis and development of laryngeal squamous cell carcinoma (LSCC), we compared the gene expression profile between matched adjacent normal tissues and LSCC tissues by cDNA microarray.

Methods: Gene expression profiles were screened using cDNA microarrays which contained the whole human being genome in related adjacent normal tissues and matched LSCC tissues from the same patients. Positive findings within the groups of seven genes invloved in cell cycle/adhesion/motility were verified with Reverse transcription-PCR (RT-PCR) and Western blot.

Results: Expression of 349 genes was significantly different between cases and controls. 112 genes were up-regulated; 237 genes were down-regulated. The results of RT-PCR and Western blot supported the reliability of our microarray analysis.

Conclusion: Our study provides the first evidence that SENP1,CD109,CKS2,Lamininα3,IntegrinαV, Integrinβ8 are up-regulated and Lamininα2 are down-regulated in LSCC. These findings provide a large body of information regarding gene expression profiles associated with LSCC, and also represent a source of potential targets for LSCC therapeutics.

Supported by: Nanjing Health Bureau Foundation