gms | German Medical Science

83rd Annual Meeting of the German Society of Oto-Rhino-Laryngology, Head and Neck Surgery

German Society of Oto-Rhino-Laryngology, Head and Neck Surgery

16.05. - 20.05.2012, Mainz

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Method-related bias in prevalence of oncogenic human papillomavirus (HPV) subtypes in head and neck squamous cell carcinoma

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  • corresponding author Ulrike Scharrer - HNO-Forschungslabor, Klinik für HNO, Uniklinikum Leipzig, Leipzig
  • Andreas Boehm - Klinik und Poliklinik für Hals-, Nasen- und Ohrenheilkunde, Universitätsklinikum, Leipzig
  • Christian Mozet - Klinik und Poliklinik für Hals-, Nasen- und Ohrenheilkunde, Universitätsklinikum, Leipzig
  • Andreas Dietz - Klinik und Poliklinik für Hals-, Nasen- und Ohrenheilkunde, Universitätsklinikum, Leipzig
  • Anett Reiche - HNO-Forschungslabor, Medizinische Fakultät, Universität Leipzig, Leipzig
  • Kristin Herrmann - HNO-Forschungslabor, Medizinische Fakultät, Universität Leipzig, Leipzig
  • Nadine Hentschel - LIFE – Leipziger Forschungszentrum für Zivilisationskrankheiten, Universität Leipzig, Leipzig
  • Solveig Tenckhoff - LIFE – Leipziger Forschungszentrum für Zivilisationskrankheiten, Universität Leipzig, Leipzig
  • Gunnar Wichmann - HNO-Forschungslabor, Medizinische Fakultät, Universität Leipzig, Leipzig

Deutsche Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie. 83. Jahresversammlung der Deutschen Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie. Mainz, 16.-20.05.2012. Düsseldorf: German Medical Science GMS Publishing House; 2012. Doc12hnod296

doi: 10.3205/12hnod296, urn:nbn:de:0183-12hnod2963

Published: April 4, 2012

© 2012 Scharrer et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.en). You are free: to Share – to copy, distribute and transmit the work, provided the original author and source are credited.

Outline

Text

Head and neck squamous cell carcinoma (HNSCC) of the oropharynx (OSCC) is known to be at least partially triggered by oncogenic subtypes of papillomaviruses (HPV). In OSCC, research mostly focused on HPV16 but essential variations in prevalence are reported. It is not yet completely clear, if these differences have to be linked to varying methods used.

Before starting HPV detection in >300 HNSCC, 100 OSCC samples of our cohort underwent analyses in five test methods based on PCR technique for validation purposes. All DNA samples were obtained from biopsies stored in TRIzol at -80°C until preparation. 100 ng each of aliquoted DNA sample underwent analyses using the following kits: BMT HPV 9G™ (BMT; Biometrix Techn. Inc.), digene HPV Genotyping RH (Qiagen), Inno-LiPA HPV (Innogenetics Inc.), Cobas™ and Linear Array™ (LA; Roche).

Different HPV detection results were obtained; e.g. for LA and BMT (two commercially available tests certified for cervical cancer) we obtained discordant results in 31/100 OSCC. Using LA, HPV was identified in 34 OSCC samples (34%); HPV16 was predominant (31 OSCC, 31%). In addition three further genotypes (HPV18, 31 and 33) were found. Using BMT, HPV-DNA was detected in 44 OSCC (44 %). However, HPV16 was just found in 22. Furthermore, one additional genotype (HPV35) was found and HPV31 was not detected. Of 100 OSCC samples, 69 showed agreement of both assays. In 45 cases both testing systems showed negative and in 24 cases concordant genotype-specific results (21 samples for HPV16).

The discordance highlights the need for standardization of HPV-DNA detection. Heterogeneity of the testing systems may be a reason for observed deviations which will be outlined in detail.

Supported by: This work was supported by LIFE-007 D9 and LIFE-006 B7.