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85th Annual Meeting of the German Society of Oto-Rhino-Laryngology, Head and Neck Surgery

German Society of Oto-Rhino-Laryngology, Head and Neck Surgery

28.05. - 01.06.2014, Dortmund

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The impact of nanoparticle solubilitiy on ROS formation and toxicity on the respiratory epithelium

Meeting Abstract

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  • corresponding author presenting/speaker Kai Fruth - HNO, HSK, Wiesbaden, Germany
  • Susanne Duca - HNO, Universitätsmedizin, Mainz, Germany
  • Anne Hilliger - HNO, HSK, Wiesbaden, Germany
  • Wolf J. Mann - HNO, Universitätsmedizin, Mainz, Germany
  • Juergen Brieger - HNO, Universitätsmedizin, Mainz, Germany

German Society of Oto-Rhino-Laryngology, Head and Neck Surgery. 85th Annual Meeting of the German Society of Oto-Rhino-Laryngology, Head and Neck Surgery. Dortmund, 28.05.-01.06.2014. Düsseldorf: German Medical Science GMS Publishing House; 2014. Doc14hno04

doi: 10.3205/14hno04, urn:nbn:de:0183-14hno044

Published: July 24, 2014

© 2014 Fruth et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.en). You are free: to Share – to copy, distribute and transmit the work, provided the original author and source are credited.

Outline

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Introduction: The respiratory epithelium is permanently exposed to metallic nanoparticles. In the present analysis we investigated the mechanism of toxicity of soluble zinc oxide (ZnO) and inert zirconium oxide (ZrO2) nanoparticles on the respiratory epithelium.

Methods: The epithelial cell line A549 was exposed to ZnO and ZrO2 nanoparticles in three different concentrations (0.1, 10, 100 µg/ml) and incubated for 4, 24, 48, and 72 h. Metabolic acitivity and ROS formation were analysed, particles’ solubility was investigated by incubation with diethylentriaminpentaacetic acid (DTPA), and electron microscope particle tracking was performed.

Results: After 24 h and 48 h exposure to ZnO but not to ZrO2 nanoparticles ROS formation was observed. Electron microscope particle tracking showed intracellular accumulation of ZrO2 but not of ZnO nanoparticles.

The decrease of cell viability after ZnO nanoparticle exposure could be diminished by diethylentriaminpentaacetic acid (DTPA).

Conclusion: These data show that toxicity of metallic nanoparticles is determined by its solubility followed by ROS formation.