gms | German Medical Science

Objectives: Skeletal Stem and Progenitor Cells (SSPCs) are at the cornerstone of bone maintenance and repair. Previous studies in mice suggest that aging is associated with a decline in SSPC number and function and therefore lending to poor skeletal health, but findings have been inconsistent. We assessed the self-renewal and differentiation potential of young and aged murine and human derived SSPCs and investigated the molecular mechanisms involved in SSPC dysfunction during aging. We hypothesize that aged SSPCs prematurely differentiate into bone, eventually leading to a loss in number and function of SSPCs in vivo with age.

Methods: Mouse: Bone marrow stromal cells from young 8–16-week-old and aged 52-82-week-old wild type (C57BL/6) mice were isolated. After flushing out the bone marrow from femurs and tibiae, cells were plated for CFU-F assays and expanded for functional assays. Human: Bone Marrow Aspirate Concentrate (BMAC) from the iliac crest of consented subjects was used. Red blood cells from collected samples were lysed. Cells were then plated for CFU-F assays and expanded for functional assays. Two-tailed Student's t-test was used to determine significant differences between data sets that are normally distributed.

Results: Bone mineral density decreases with age, and thus it is widely thought that SSPC osteogenic potential is also reduced. Unexpectedly, we observed that, while aged SSPCs exhibit a reduced self-renewal capacity compared to their young counterparts, they produce significantly more bone in vitro. To determine the molecular mechanisms that drive this change, we performed RNA-sequencing of young and aged SSPCs and identified several components of key osteogenic signaling pathways (BMP & WNT) that are upregulated during aging. However, when young and aged SSPCs are transplanted under the renal capsule in vivo, young SSPCs prove to be more osteogenic.

Conclusion: In vivo, bone formation declines with age. It is largely thought that this is due to a loss of the osteogenic capacity of SSPCs. We show that aged SSPCs in vitro are more osteogenic, whereas young SSPCs are more osteogenic in vivo, suggesting a model whereby native SSPC in vivo functionality is perturbed in vitro. We have identified BMP & WNT signaling pathways as therapeutic interventions to recalibrate the balance between self-renewal and differentiation to replenish the aged SSPC pool and rejuvenate the aged skeleton.