Article
Altered expression of the adhesion GPCRs EMR2/ADGRE2 and CD97/ADGRE5 at circulating innate immune cells after traumatic injury
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Authors
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Leyu Zheng
- University of Leipzig, Research Laboratory of Trauma, Leipzig, Germany
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Moujie Rang
- University of Leipzig, Research Laboratory of Trauma, Leipzig, Germany
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Carolin Fuchs
- University of Leipzig, Research Laboratory of Trauma, Leipzig, Germany
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Cheng-Chih Hsiao
- University of Amsterdam, Academic Medical Center, Amsterdam, Netherlands
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Annette Keß
- University of Leipzig, Research Laboratory of Trauma, Leipzig, Germany
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Christian Kleber
- University of Leipzig, Research Laboratory of Trauma, Leipzig, Germany
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Georg Osterhoff
- University of Leipzig, Research Laboratory of Trauma, Leipzig, Germany
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Gabriela Aust
- University of Leipzig, Research Laboratory of Trauma, Leipzig, Germany
| Published: | October 23, 2023 |
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Outline
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Objectives: The time course and clinical outcome of severely injured patients are determined by the triggered inflammatory and compensatory anti-inflammatory and resolving immune responses. Innate immune cells provide a first line defense to the damage-associated molecular pattern (DAMP) released after major trauma. EMR2/ADGRE2 and CD97/ADGRE5, members of the Adhesion G protein-coupled receptor (aGPCR) family, are expressed at circulating neutrophils and monocytes und involved in their regulation.
Methods: Here, we monitored the expression of EMR2 and CD97 at circulating leukocytes of injured patients (injury severity score, ISS 9-75, n=34) up to 240 h after trauma by multicolor flow cytometry, and related the data to clinical and pathophysiological parameters of the patients.
Results and conclusion: The amount of immature neutrophils is higher in polytraumatized patients with an ISS >=25 (n=25) compared to less-injured patients (ISS <25; n=9) one and 8 h after injury. Interestingly, 8 h after trauma the percentage of EMR2+ classical and intermediate monocytes is decreased in patients with an ISS >=25, but not in less-injured patients. This early downregulation of EMR2 at monocytes correlates to increased levels of inflammatory cytokines. Further, the usually low percentage of EMR2+ mature neutrophilic granulocytes increased obviously 24–48 h and normalized 120–240 h after trauma. This characteristic time course was seen in almost all patients independent of the ISS, but not in healthy controls (n=5). In sum, aGPCR are regulated after polytraumatic injury at innate immune cells and are likely potential biomarkers for the patient's time course after trauma.
