Article
Increased functional abnormalities in neutrophils are caused by a phenotypic shift of three different subsets after trauma
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Authors
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Andrea Janicová
- Johann Wolfgang Goethe Universität Frankfurt am Main, Klinik für Unfall-, Hand- und Wiederherstellungschirurgie, Frankfurt am Main, Germany
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Baolin Xu
- Johann Wolfgang Goethe Universität Frankfurt am Main, Klinik für Unfall-, Hand- und Wiederherstellungschirurgie, Frankfurt am Main, Germany
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Philipp Störmann
- Johann Wolfgang Goethe Universität Frankfurt am Main, Klinik für Unfall-, Hand- und Wiederherstellungschirurgie, Frankfurt am Main, Germany
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Ingo Marzi
- Johann Wolfgang Goethe Universität Frankfurt am Main, Klinik für Unfall-, Hand- und Wiederherstellungschirurgie, Frankfurt am Main, Germany
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Borna Relja
- Johann Wolfgang Goethe Universität Frankfurt am Main, Klinik für Unfall-, Hand- und Wiederherstellungschirurgie, Frankfurt am Main, Germany
| Published: | October 22, 2019 |
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Outline
Text
Objectives: Severely injured patients (TP) are prone to the development of secondary infectious complications due to imbalanced immune response. Neutrophils as a first line defence against invading pathogens were recently subdivided into three subsets with possible functional diversity. Since phenotypic and functional abnormalities are not fully characterized after trauma, we analysed dynamic changes and production of reactive oxygen species (ROS) and the phagocytic capacity in each neutrophil subset in TP.
Methods: Peripheral blood was withdrawn from TP (n=12) at day 1 after trauma and from healthy volunteers (HV) and stained for CD16 and CD62L. CD16dimCD62Lbright (immature), CD16brightCD62Lbright (mature) and CD16brightCD62Ldim ("aged", „immune-suppressive") neutrophil phenotypes were assessed by flow cytometry. Subsequently, oxidative burst activity and phagocytic capacity were analysed.
Results and conclusion: Immature neutrophils became significantly more abundant in trauma, whereas mature subset stayed stable and „aged“ phenotype displayed a slight decrease. ROS production in TP vs. HV increased significantly in CD16+ granulocytes. Individually, immature neutrophils of TP showed no changes in production of ROS, while mature and „aged“ phenotypes exerted increased ROS levels. Nevertheless, the highest ROS production was measured in immature neutrophil subset. Phagocytic capacity increased in CD16+ granulocytes of TP vs. HV among the stable amount of phagocyting cells. Whereas all neutrophil subsets displayed increase of phagocytosis, the highest intake of E. coli bioparticle was in „aged“ neutrophils.
These data show a phenotypic shift in neutrophil subsets after trauma. Increased functional abnormalities are caused by certain phenotypes, suggesting differential antimicrobial functions of neutrophil subsets after trauma. Taken together, aberrant neutrophil functionality post-trauma may contribute to development of immune response disbalance, and modulation of those may represent a potential therapeutic target.
