gms | German Medical Science

German Congress of Orthopaedics and Traumatology (DKOU 2019)

22. - 25.10.2019, Berlin

Improved in vitro colonization of a cell-free collagen type I scaffold achieved by a feasible method of matrix modification

Meeting Abstract

  • presenting/speaker Philip Roessler - Klinik und Poliklinik für Orthopädie und Unfallchirurgie, Universitätsklinikum Bonn, Bonn, Germany
  • Caecilia Hilgers - Klinik und Poliklinik für Orthopädie und Unfallchirurgie, Universitätsklinikum Bonn, Bonn, Germany
  • Werner Masson - Klinik und Poliklinik für Orthopädie und Unfallchirurgie, Universitätsklinikum Bonn, Bonn, Germany
  • Thomas Randau - Klinik und Poliklinik für Orthopädie und Unfallchirurgie, Universitätsklinikum Bonn, Bonn, Germany
  • Dieter C. Wirtz - Klinik und Poliklinik für Orthopädie und Unfallchirurgie, Universitätsklinikum Bonn, Bonn, Germany
  • Frank Schildberg - Klinik und Poliklinik für Orthopädie und Unfallchirurgie, Universitätsklinikum Bonn, Bonn, Germany

Deutscher Kongress für Orthopädie und Unfallchirurgie (DKOU 2019). Berlin, 22.-25.10.2019. Düsseldorf: German Medical Science GMS Publishing House; 2019. DocAB21-1395

doi: 10.3205/19dkou097, urn:nbn:de:0183-19dkou0977

Published: October 22, 2019

© 2019 Roessler et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution 4.0 License. See license information at http://creativecommons.org/licenses/by/4.0/.


Outline

Text

Objectives: Cell-free cartilage repair has come a long way and has been accepted as a standard treatment option besides MACT, especially in Europe during recent years. However, mechanisms of colonization are still poorly understood and thus cellular origin in repair tissues remains subject of debate. Adjacent cartilage, synovium and subchondral bone are among the possible cell sources. Moreover, there is an ongoing search for possible matrix modifications to increase the cell yields of this method. The aim of this study was to investigate the colonization potential of different adult stem cell niches on a modified collagen type I scaffold under different culture conditions.

Methods: Human mesenchymal stromal cells (MSC) were isolated and expanded from cartilage (C-MSC), synovial fluid (S-MSC) and bone marrow aspirate (BM-MSC). Cells were seeded on a collagen type I scaffold (CaReS-1S, ArthroKinetics GmbH, Krems, Austria) and cultivated with either chondroinductive or standard DMEM high Gluc (control) media. Secondly, scaffold surfaces were either perforated with a custom-made micro needle device or left intact, generating two groups with two subgroups each. After 21 days of culture, scaffolds were fixed and processed for fluorescence and confocal microscopy to assess cell migration, densities and scaffold morphology.

Results and conclusion: The collagen type I matrix did not show a significant preference for any MSC type used under chondroinductive or control conditions. Induction-hampered cell migration into the scaffold, limiting more than 90% of relative cell counts to 0-40 µm, whereas controls showed a generally deeper migration up to 60-80 µm. Perforation of the scaffold surface resulted in a central ingrowth, mainly along the generated canals under inductive conditions, whereas controls showed a much broader ingrowth and a greater migration distance from the canals into the matrix. Increased aggrecan synthesis on the surface was observed in all specimens under inductive, but not under control conditions. This feasible method of matrix modification using a micro needle device led to a significant improvement in cell migration and matrix colonization. These results will have to be confirmed in an in vivo animal model to prove their efficacy for a possible clinical application.