gms | German Medical Science

49. Jahrestagung der Deutschen Gesellschaft für Plastische und Wiederherstellungschirurgie (DGPW)

Deutsche Gesellschaft für Plastische und Wiederherstellungschirurgie e. V.

06.10.-08.10.2011, Ulm

LOH as predictive markers and for verification of neoplasticity/identity of cultured tumor cells

Meeting Abstract

  • corresponding author Lan Kluwe - Uniklinik Hamburg-Eppendorf, Klinik und Poliklinik für Mund-, Kiefer- und Gesichtschirurgie, Hamburg
  • Melanie Spyra - Uniklinik Hamburg-Eppendorf, Klinik und Poliklinik für Mund-, Kiefer- und Gesichtschirurgie, Hamburg; Forschungslabor MKG, Hamburg
  • Theresa Häger - Uniklinik Hamburg-Eppendorf, Klinik und Poliklinik für Mund-, Kiefer- und Gesichtschirurgie, Hamburg; Forschungslabor MKG, Hamburg
  • Markus Freytag - Uniklinik Hamburg-Eppendorf, Klinik und Poliklinik für Mund-, Kiefer- und Gesichtschirurgie, Hamburg; Forschungslabor MKG, Hamburg
  • Victor-Felix Mautner - Uniklinik Hamburg-Eppendorf, Klinik und Poliklinik für Neurologie, Hamburg
  • Max Heiland - Uniklinik Hamburg-Eppendorf, Klinik und Poliklinik für Mund-, Kiefer- und Gesichtschirurgie, Hamburg
  • Marco Blessmann - Uniklinik Hamburg-Eppendorf, Klinik und Poliklinik für Mund-, Kiefer- und Gesichtschirurgie, Hamburg

Deutsche Gesellschaft für Plastische und Wiederherstellungschirurgie. 49. Jahrestagung der Deutschen Gesellschaft für Plastische und Wiederherstellungschirurgie (DGPW). Ulm, 06.-08.10.2011. Düsseldorf: German Medical Science GMS Publishing House; 2011. Doc11dgpw046

doi: 10.3205/11dgpw046, urn:nbn:de:0183-11dgpw0466

Published: December 7, 2011

© 2011 Kluwe et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.en). You are free: to Share – to copy, distribute and transmit the work, provided the original author and source are credited.


Outline

Text

Loss of heterozygosity (LOH), is a frequent genetic events in tumor and can be used as predictive marker for tumor development and progression. For example, LOH of chromosomes 3p and 9p are among the most predictive markers for oral squamous cell carcinomas (OSCC). On the other hand, LOH analysis provides a strategy to verify the neoplasticity and identity of cells derived from tumors. LOH in tumors can be examined by typing linked microsatellite markers in paired blood/tumor specimen and in tumor-derived cells. LOH-analysis enables verification of neoplastic authenticity of cells in cultures. We have found that cells derived from tumors, also from malignant tumors, are frequently not tumor cells, but often non-tumor cells, e.g., fibroblasts. Furthermore, genotyping using the same microsatellite markers provides genetic finger prints and thus enables detection of cross-contamination which occurs more often than is expected. In future studies, we will fine map LOH on chromosome 3p and 9p in OSCC using 30-40 microsatellite markers. Markers frequently showing LOH in these tumors will be evaluated for their predictive value for development and progress of OSCC. These markers will also be used to genotype cells derived from OSCC, enabling verification of neoplasticity and identity of the cells in culture.