Artikel
LOH as predictive markers and for verification of neoplasticity/identity of cultured tumor cells
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Veröffentlicht: | 7. Dezember 2011 |
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Gliederung
Text
Loss of heterozygosity (LOH), is a frequent genetic events in tumor and can be used as predictive marker for tumor development and progression. For example, LOH of chromosomes 3p and 9p are among the most predictive markers for oral squamous cell carcinomas (OSCC). On the other hand, LOH analysis provides a strategy to verify the neoplasticity and identity of cells derived from tumors. LOH in tumors can be examined by typing linked microsatellite markers in paired blood/tumor specimen and in tumor-derived cells. LOH-analysis enables verification of neoplastic authenticity of cells in cultures. We have found that cells derived from tumors, also from malignant tumors, are frequently not tumor cells, but often non-tumor cells, e.g., fibroblasts. Furthermore, genotyping using the same microsatellite markers provides genetic finger prints and thus enables detection of cross-contamination which occurs more often than is expected. In future studies, we will fine map LOH on chromosome 3p and 9p in OSCC using 30-40 microsatellite markers. Markers frequently showing LOH in these tumors will be evaluated for their predictive value for development and progress of OSCC. These markers will also be used to genotype cells derived from OSCC, enabling verification of neoplasticity and identity of the cells in culture.