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46. Jahrestagung der Deutschen Gesellschaft der Plastischen, Rekonstruktiven und Ästhetischen Chirurgen (DGPRÄC), 20. Jahrestagung der Vereinigung der Deutschen Ästhetisch-Plastischen Chirurgen (VDÄPC)

01.10. - 03.10.2015, Berlin

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The Influence of Ultrasound Assisted Liposuction on the Regenerative Potential of Adipose Derived Stem Cells

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  • presenting/speaker Dominik Duscher - Johannes Kepler Universität Linz, Österreich
  • David Atashroo - Stanford University, CA, USA
  • Zeshaan Maan - Stanford University, CA, USA
  • Anna Luan - Stanford University, CA, USA
  • Janos Barrera - Stanford University, CA, USA
  • Alexander Whittam - Stanford University, CA, USA
  • Michael Hu - Stanford University, CA, USA
  • Graham Walmsley - Stanford University, CA, USA
  • Michael Pollhammer - Johannes Kepler Universität Linz, Österreich
  • Manfred Schmidt - Johannes Kepler Universität Linz, Österreich
  • Georg Huemer - Johannes Kepler Universität Linz, Österreich
  • Michael Longaker - Stanford University, CA, USA
  • Geoffrey Gurtner - Stanford University, CA, USA

Deutsche Gesellschaft der Plastischen, Rekonstruktiven und Ästhetischen Chirurgen. Vereinigung der Deutschen Ästhetisch-Plastischen Chirurgen. 46. Jahrestagung der Deutschen Gesellschaft der Plastischen, Rekonstruktiven und Ästhetischen Chirurgen (DGPRÄC), 20. Jahrestagung der Vereinigung der Deutschen Ästhetisch-Plastischen Chirurgen (VDÄPC). Berlin, 01.-03.10.2015. Düsseldorf: German Medical Science GMS Publishing House; 2015. Doc098

doi: 10.3205/15dgpraec098, urn:nbn:de:0183-15dgpraec0989

Published: September 28, 2015

© 2015 Duscher et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution 4.0 License. See license information at http://creativecommons.org/licenses/by/4.0/.

Outline

Text

Introduction: Human adipose derived stem cells (ASCs) have gained recent interest for their therapeutic potential in regenerative medicine. Even though liposuction is the primary method of obtaining ASCs, little is known about its effects on ASC regenerative abilities. Ultrasound assisted liposuction (UAL) can facilitate the process of lipoaspiration by increasing the speed and safety of the fat harvest. However, a paucity of evidence exists regarding its impact on the regenerative capacity of harvested ASCs. In this study, we evaluate the regenerative abilities of ASCs harvested with a third generation UAL device versus the industry standard suction assisted lipoaspiration (SAL) and ASCs isolated from excised fat.

Methods: Fat samples were obtained from elective surgery patients via UAL or SAL and abdominoplasty. The tissue was processed for enzymatic isolation of the stromal vascular fraction (SVF). The SVF was then sorted using Fluorescent Assisted Cell Sorting (FACS) based on an established progenitor surface marker profile (CD34+CD31-CD45-) and live/dead gating, to obtain viable ASCs. An MTT assay was performed on cultured ASCs to compare their proliferation potential. The multlineage differentiation capacity of the ASCs was then assessed by the induction of adipogenic and osteogenic differentiation and subsequent Oil Red O, Alkaline Phosphatase and Alizarin Red staining. Additionally, qRT-PCR expression analysis of key adipogenic and osteogenic genes was performed at culture day 0, 7, and 14. Finally, the regenerative potential of the ASCs was compared in and in vivo wound healing model.

Results: UAL and SAL lipoaspirates demonstrated a reduced viable ASC yield on FACS compared to excisional fat, however equivalent proliferative potential on MTT assay (*p > .05). There was no significant difference in adipogenic or osteogenic differentiation capacity (*p > .05). Likewise, qRT-PCR showed similar expression of multiple osteogenic and adipogenic genes in all ASC groups (*p > .05). Wound healing was significantly improved in all cell therapy groups compared to the control group (*p .05). This could further be confirmed on a histological level by an equally significant enhancement of neovascularization in the cell therapy groups.

Conclusion: UAL represents a successful method for obtaining ASCs for regenerative medicine. Cells harvested with this alternative approach to liposuction are suitable for potential therapeutic and tissue engineering applications.