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46. Jahrestagung der Deutschen Gesellschaft der Plastischen, Rekonstruktiven und Ästhetischen Chirurgen (DGPRÄC), 20. Jahrestagung der Vereinigung der Deutschen Ästhetisch-Plastischen Chirurgen (VDÄPC)

01.10. - 03.10.2015, Berlin

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Comparison of the effect of negative pressure wound therapy with different antiseptic dressings on Gr+ and Gr- bacteria in experimental in-vitro wounds

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  • presenting/speaker Johannes Matiasek - Wilhelminenspital der Stadt Wien, Österreich
  • Konrad Domig - BOKU - Universität für Bodenkultur Wien, Österreich
  • Ojan Assadian - University of Huddersfield, UK

Deutsche Gesellschaft der Plastischen, Rekonstruktiven und Ästhetischen Chirurgen. Vereinigung der Deutschen Ästhetisch-Plastischen Chirurgen. 46. Jahrestagung der Deutschen Gesellschaft der Plastischen, Rekonstruktiven und Ästhetischen Chirurgen (DGPRÄC), 20. Jahrestagung der Vereinigung der Deutschen Ästhetisch-Plastischen Chirurgen (VDÄPC). Berlin, 01.-03.10.2015. Düsseldorf: German Medical Science GMS Publishing House; 2015. Doc048

doi: 10.3205/15dgpraec048, urn:nbn:de:0183-15dgpraec0488

Published: September 28, 2015

© 2015 Matiasek et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution 4.0 License. See license information at http://creativecommons.org/licenses/by/4.0/.

Outline

Text

Negative pressure wound therapy (NPWT) has established successfully as a treatment strategy to support wound healing in a number of clinical indications. However, systematic research investigating the bacterial kinetics on wounds is lacking and just a few studies are available comparing the microbiological difference of antiseptic dressings or rinsing solutions in combination with NPWT. The aim of this study was to investigate the antimicrobial effect of different antiseptic options (dressing or rinsing solution) in combination with negative pressure wound therapy on standardized experimental porcine wounds colonized with Gr+ or Gr- bacteria (Staphylococcus aureus or Escherichia coli).

Wounds were treated with a NPWT system capable to instill liquid solutions (V.A.C. UltaTM), and negative pressure was applied in combination with antiseptic dressings (silver, polyhexanide) or intermitted instillation of a wound irrigation solution (octenidine). The device was operated at 125 mm Hg sub-atmospheric pressure. In the silver and polyhexanide group continuous negative pressure was applied, whereas in the octenidine group automated intermittent instillation for about 3 minutes every 240 minutes were performed. Bacterial load per gram tissue were measured before intervention and after 24 or 48 hours, respectively.

In this artificial wound model a significant reduced bacterial growth compared to traditional NPWT was observed after 48 hours, when silver dressings or instillation with octenidine were used.