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68th Annual Meeting of the German Society of Neurosurgery (DGNC)
7th Joint Meeting with the British Neurosurgical Society (SBNS)

German Society of Neurosurgery (DGNC)

14 - 17 May 2017, Magdeburg

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Characterization of an intracranial rat model for local treatment

Meeting Abstract

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  • Zhiqun Wu - Medizinische Hochschule Hannover, Neurochirurgische Klinik, Hannover, Deutschland
  • Kerstin Schwabe - Medizinische Hochschule Hannover, Neurochirurgische Klinik, Hannover, Deutschland
  • Joachim K. Krauss - Medizinische Hochschule Hannover, Neurochirurgische Klinik, Klinik für Neurochirurgie, Hannover, Deutschland
  • Makoto Nakamura - Kliniken der Stadt Köln, Klinikum Merheim, Neurochirurgische Klinik, Köln, Deutschland
  • Nadine John - Medizinische Hochschule Hannover, Neurochirurgische Klinik, Hannover, Deutschland

Deutsche Gesellschaft für Neurochirurgie. Society of British Neurological Surgeons. 68. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie (DGNC), 7. Joint Meeting mit der Society of British Neurological Surgeons (SBNS). Magdeburg, 14.-17.05.2017. Düsseldorf: German Medical Science GMS Publishing House; 2017. DocP 052

doi: 10.3205/17dgnc615, urn:nbn:de:0183-17dgnc6156

Published: June 9, 2017

© 2017 Wu et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution 4.0 License. See license information at http://creativecommons.org/licenses/by/4.0/.

Outline

Text

Objective: Glioblastoma multiforme (GBM) is the most frequent malignant primary brain tumor. Despite advances in microsurgical techniques and concomitant radiochemotherapy, the overall prognosis for patients is still poor. In this study we characterized a syngeneic, orthotopic glioblastoma rat model based on the BT4Ca cell line for its use to test local treatment, either by direct injection into the tumor or after tumor resection into the resection cavity.

Methods: BT4Ca cells were stereotactically implanted into the prefrontal cortex of BDIX rats. In the first group (n= 22 rats) a guiding cannula was implanted aiming at the implantation site, followed by microinjection of vehicle (here 10% dimethylsulfoxid in phosphate buffered saline) every three days via the implanted guiding cannula. In the second group (n= 18 rats) the tumor was resected 8 days after cell injection and vehicle given into the resection cavity. A third group (n= 12 rats) with implanted tumor cells but without further manipulation served as control. Postoperatively the survival time and health conditions were scored for humane endpoint criterion. The tumor growth was histologically verified.

Results: Implantation of BT4Ca cells reliably induced fast growing tumors with a mean survival time of 16 days and a tumor induction rate of 94.2%. In all groups the health score and body weight only deteriorated 1-2 days before humane endpoint criterion. Implantation of a guide cannula and microinjection of vehicle did not affect the survival time. The survival time after tumor resection with concomitant local application of vehicle was similar to that of the control group (i.e., in mean 16 days after resection). The tumor histology was similar in all groups.

Conclusion: We here present a fast and robust intracranial rat glioblastoma model that could be used to test the anti-tumor effects of local therapeutic strategies aiming directly into the tumor or applied locally after tumor resection under standardized experimental conditions.