Article
"Two are never enough"- Impact of the number of tissue samples taken in stereotactic biopsy
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Published: | June 9, 2017 |
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Objective: Stereotactic procedures are performed in many neurosurgical departments in order to obtain tumor tissues from brain lesions for histopathological evaluation. It is still unclear how many tissue samples have to be taken to establish a final diagnosis based on histopathological and genetic examinations. Only histopathological diagnosis results in adequate therapy.
Methods: We included 43 consecutive patients who underwent stereotactic biopsy of a suspected glioblastoma between 02/2013 and 07/2015. All patients showed contrast enhancing tumors in the MRI. The patients underwent stereotactic biopsy with the Leksell frame attached to their head. Target and Entry Points were calculated with BrainLab iplan software (BrainLab iplan 1.0, Feldkirchen, Germany). First the two samples 5mm before the Target (pre-target) and the “Targetpoint” itself were analyzed (group 1), then a histopathological evaluation of all samples was performed (group 2).
Results: Regarding all patients, a median of 14 samples were taken. Using hematoxilin-eosin staining a correct histopathological diagnosis was made in only 30 cases of group 1. In detail a correct diagnosis was made in 73% of the glioblastoma patients, in none of the anaplastic astrocytoma patients, in 100% of diffuse glioma patients, and in 100% of the carcinoma patients. In 3 cases of group 1 only necrosis was found. Contrariwise a final diagnosis was made in 100% of the patients of group 2.
Conclusion: For patients with suspected glioblastoma, a minimum sample number of ten (4-6 samples for hematoxilin-eosin staining and 4 for molecular diagnosis) should be taken for histopathologic and genetic examination in order to establish a final diagnosis.