gms | German Medical Science

64th Annual Meeting of the German Society of Neurosurgery (DGNC)

German Society of Neurosurgery (DGNC)

26 - 29 May 2013, Düsseldorf

Neuroprotective efficacy of prophylactically administered nimodipine in PC12 cells exposed to different types of stress

Meeting Abstract

  • Kaya Bork - Institute of Physiological Chemistry, University of Halle-Wittenberg, Germany
  • Franziska Wurm - Institute of Physiological Chemistry, University of Halle-Wittenberg, Germany; Department of Neurosurgery, University of Halle-Wittenberg, Germany
  • Rüdiger Horstkorte - Institute of Physiological Chemistry, University of Halle-Wittenberg, Germany
  • Sebastian Simmermacher - Department of Neurosurgery, University of Halle-Wittenberg, Germany
  • Christian Strauss - Department of Neurosurgery, University of Halle-Wittenberg, Germany
  • Christian Scheller - Department of Neurosurgery, University of Halle-Wittenberg, Germany

Deutsche Gesellschaft für Neurochirurgie. 64. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie (DGNC). Düsseldorf, 26.-29.05.2013. Düsseldorf: German Medical Science GMS Publishing House; 2013. DocMO.09.02

doi: 10.3205/13dgnc072, urn:nbn:de:0183-13dgnc0723

Published: May 21, 2013

© 2013 Bork et al.
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Outline

Text

Objective: Though the neuroprotective effect of nimodipine is proven following both subarachnoidal hemorrhage (SAH) and vestibular schwannoma (VS) surgery, its underlaying mechanism is still unknown. The dependency of its neuroprotective efficacy on different types of cellular stress was investigated in a model using pheochromocytoma (PC12) cells which have similar properties as compared with neuronal cells. The chosen modes of stress should simulate potential intraoperative mechanisms of neuronal damages caused by mechanical maneuvers and changes of the microenvironment of the neuronal tissue.

Method: PC12 cells were treated with nimodipine for 48h before exposing them to alcoholic (200mM EtOH), osmotic (30mM NaCl-solution) and mechanical stress. The controls did not receive nimodipine prophylaxis. Mechanical-stress was imitated by a scratch-assay. Thereafter, the cells were additionally incubated for 48 h. Cell death was measured by quantifying the activity of released lactat dehydrogenase (LDH) into the medium. LDH activity was determined spectrophotometrically at 340 nm by following the NADH oxidation. Statistical significance was determined by t-test for independent samples.

Results: The LDH release into the culture medium of the cells with nimodipine prophylaxis stressed by alcohol (p=0.01) and NaCl-solution (p=0.02) were statistical significantly lower than the LDH release of the controls. Additionally, there was a trend for a lower LDH release in the mechanically stressed cells which received the nimodipine prophylaxis as compared with the controls.

Conclusions: The results point to a general neuroprotective efficacy of prophylactically administered nimodipine. Neuroprotective effects of nimodipine were observed after alcoholic, osmotic and mechanical stress in the presented study and after oxygen-glucose deprivation in the literature. Consequently neuroprotection induced by prophylactically administered nimodipine may be effective independently from the type of cellular stress.