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133. Kongress der Deutschen Gesellschaft für Chirurgie

Deutsche Gesellschaft für Chirurgie

26.04. - 29.04.2016, Berlin

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Targeting hypoxia as novel therapeutic approach in treatment of neuroblastoma in vitro

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  • Stephanie Gros - Universitäts-Kinderspital beider Basel, Kinderchirurgie, Basel, Switzerland
  • Urs Kym - Universitäts-Kinderspital beider Basel, Kinderchirurgie, Basel, Switzerland
  • Viktoria Pfeifle - Universitäts-Kinderspital beider Basel, Kinderchirurgie, Basel, Switzerland
  • Claudiu Supuran - Sezione di Scienze farmaceutiche, University of Florence, Department Neurofarba, Florenz, Italy
  • Stefan Holland-Cunz - Universitäts-Kinderspital beider Basel, Kinderchirurgie, Basel, Switzerland

Deutsche Gesellschaft für Chirurgie. 133. Kongress der Deutschen Gesellschaft für Chirurgie. Berlin, 26.-29.04.2016. Düsseldorf: German Medical Science GMS Publishing House; 2016. Doc16dgch621

doi: 10.3205/16dgch621, urn:nbn:de:0183-16dgch6214

Published: April 21, 2016

© 2016 Gros et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution 4.0 License. See license information at http://creativecommons.org/licenses/by/4.0/.

Outline

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Background: Several oxygen dependent factors including CAIX (carbonic anhydrase IX) and phosphoglycerate kinase 1 (PGK1) have previously been shown to be involved in processes of tumor pathology including proliferation, metastases and poor prognosis in neuroblastoma patients. The aim of the current study was to evaluate the therapeutic potential of targeting oxygen dependent proteins in neuroblastoma cell lines in vitro.

Materials and methods: Expression profile for oxygen dependent factors including CAIX and PGK1 was generated using a panel of neuroblastoma cell lines. Cell lines with or without elevated RNA and protein expression levels were treated with specific inhibitors and proliferation and migration abilities were assessed.

Results: Expression of hypoxia dependent factors was found in all neuroblastoma cell lines. CAIX and PGK1 expression was up regulated under hypoxia to different extents. Proliferation and migration were significantly impaired by targeted inhibition of these enzymes. CAIX hereby appears to be the stronger prognostic indicator.

Conclusion: Hypoxic factors in the tumor cell`s microenvironment further proliferation and migration in vitro. This strengthens the perspectives for additive novel therapeutic approaches targeting hypoxia-dependent factors in this childhood disease.