gms | German Medical Science

133. Kongress der Deutschen Gesellschaft für Chirurgie

Deutsche Gesellschaft für Chirurgie

26.04. - 29.04.2016, Berlin

Article

Send article

Cell reflect ampullary cancer subtypes

Meeting Abstract

Search Medline for

  • Louisa Bolm - UKSH Campus Lübeck, Clinic for Surgery, Lübeck, Deutschland
  • Zon Weng Lai - University of Freiburg, Institute of Molecular Medicine and Cell Research, Freiburg, Deutschland
  • Peter Bronsert - University Medical Center Freiburg, Institute of Pathology, Freiburg, Deutschland
  • Dirk Bausch - UKSH Campus Lübeck, Clinic for Surgery, Lübeck, Deutschland
  • Tobias Keck - UKSH Campus Lübeck, Clinic for Surgery, Lübeck, Deutschland
  • Oliver Schilling - University of Freiburg, Institute of Molecular Medicine and Cell Research, Freiburg, Deutschland
  • Ulrich Friedrich Wellner - UKSH Campus Lübeck, Clinic for Surgery, Lübeck, Deutschland

Deutsche Gesellschaft für Chirurgie. 133. Kongress der Deutschen Gesellschaft für Chirurgie. Berlin, 26.-29.04.2016. Düsseldorf: German Medical Science GMS Publishing House; 2016. Doc16dgch231

doi: 10.3205/16dgch231, urn:nbn:de:0183-16dgch2316

Published: April 21, 2016

© 2016 Bolm et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution 4.0 License. See license information at http://creativecommons.org/licenses/by/4.0/.

Outline

Text

Background: Ampullary cancer is a relatively rare entity and usually treated by pancreatoduodenectomy followed by adjuvant therapy. The intestinal subtype is associated with markedly improved prognosis after resection. Only few cell lines are available for in vitro studies of ampullary cancer and they have not been collectively characterized.

Materials and methods: We characterized available ampullary cancer cell lines by subtype maker expression, epithelial-mesenchymal transition (EMT) features, growth and invasion, drug sensitivity and response to cancer-associated fibroblast conditioned medium (CAF-CM).

Results: On the basis of EMT features, subtype marker expression, growth, invasion and drug sensitivity three types of cell lines could be distinguished: mesenchymal-like, pancreatobiliary-like and intestinal-like. In response to CAF-CM, enhanced growth, EMT induction as well as suppression of intestinal differentiation markers were observed, but in a heterogenous pattern. Also proteomic analysis of the CAF response distinguished intestinal-like from other cell lines.

Conclusion: Most of the available AMPAC cell lines seem to reflect a poorly differentiated pancreatobiliary or mesenchymal-like phenotype, consistent with their origin. We suggest that the best cell line model for intestinal-like AMPAC is the SNU869 cell line, while others seem to reflect aggressive AMPAC subtypes.