Artikel
The induction of CXCR4 expression in human osteoblast-like cells (MG63) by CoCr particles is regulated by the PLC-DAG-PKC pathway
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Autoren
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Andreas Drynda
- Otto-von-Guericke Universität Magdeburg, Orthopädische Universitätsklinik, Magdeburg, Germany
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Qiang Ren
- Otto-von-Guericke Universität Magdeburg, Orthopädische Universitätsklinik, Magdeburg, Germany
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Gottfried H. Buchhorn
- Department of Orthopaedics,, University Medical Center, Göttingen, Göttingen, Germany
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Christoph H Lohmann
- Otto-von-Guericke Universität Magdeburg, Orthopädische Universitätsklinik, Magdeburg, Germany
| Veröffentlicht: | 10. Oktober 2016 |
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Gliederung
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Background: Osteolysis which leads to aseptic loosening of implants is a fundamental problem in joint replacement surgery (arthroplasty) and is the leading cause for implant failure and revision surgery. Metal (Co-28Cr-6Mo) particles separated from implants by wear cause osteolysis and the failure of orthopedic implants, but the molecular mechanism is not clear. The chemokine receptor CXCR4 has been shown to play a pivotal role in periprosthetic osteolysis. It was the aim of the study to determine which signal transduction pathway (PLC-DAG-PKC or MAPK/ERK) induces CXCR4 expression in osteoblast-like cells (MG63) cells.
Methods: MG63 and Jurkat cells were stimulated with different amounts of particles [1 x E7, 1 x E6, and 1 x E5] for different time periods (30 min to 24 h), in the presence and absence of specific inhibitors (chelerythrine for the PLC-DAG-PKC pathway and PD98059 for the MAPK/ERK pathway). The expression of CXCR4 specific mRNA was determined by Real-Time PCR, PKC activity was measured by Western Blot using an antibody specific for PKC related phosphorylation.
Results: Real-Time PCR data showed that CXCR4 mRNA expression in MG63 cells induced by CoCr particles was significantly diminished by the PKC specific inhibitor chelerythrine. This effect was not observed with the MAPK/ERK inhibitor PD98059. The involvement of PKC was also confirmed by an intensified phosphorylation pattern after stimulation with CoCr particles. In Jurkat cells none of the inhibitors exhibited any effect.
Conclusion: The induction of CXCR4 specific mRNA expression in MG63 cells after stimulation with CoCr particles is regulated by the PLC-DAG-PKC pathway and not by the MAPK/ERK pathway.
