Artikel
SDF-1α expression in skin tissue can be upregulated by mechanical stretch and recruit circulating bone marrow-derived stem cells toward expanded skin
Suche in Medline nach
Autoren
-
Shuangbai Zhou
- Shanghai, China
-
Qing-Feng Li
- Shanghai Jiao Tong University, Shanghai Ninth People\’s Hospital, Shanghai, China
-
Cheng-An Chiang
- Shanghai, China
-
Jing Wang
- Shanghai Jiao Tong University, Shanghai Ninth People\’s Hospital, Shanghai, China
-
Yun Xie
- Shanghai Jiao Tong University, Shanghai Ninth People\’s Hospital, Shanghai, China
-
Tao Zan
- Shanghai Jiao Tong University, Shanghai Ninth People\’s Hospital, Shanghai, China
-
Kai Liu
- Shanghai, China
| Veröffentlicht: | 3. September 2014 |
|---|
Gliederung
Text
Question: Skin expansion is a procedure that stimulates skin regeneration by applying continuous mechanical stretching of normal donor skin for reconstruction purposes. However, it is unclear how circulating bone marrow-derived mesenchymal stem cells (MSCs) respond to mechanical stretch in skin tissue and participate in skin regeneration.
Methods: MSCs from luciferase-Tg Lewis rats were transplanted into a rat tissue expansion model and tracked in vivo continuously by luminescence imaging to observe MSCs migration during skin expansion. Expression levels of chemokines were evaluated in mechanically stretched tissues, as were their related chemokine receptors in MSCs. Chemotactic assays were conducted in vitro and in vivo to assess the impact of chemokine expression on MSC migration. Expanded skin sections were stained with anti-luciferase antibody, to mark migrated MSCs, and anti-K19, CD31 antibodies, to analyze differentiation of migrated MSCs. The effect of migrated MSCs in enhancing skin regeneration was evaluated by in vivo blocking chemokine/chemokine receptor pathway.
Results: MSC migration was observed in mechanically stretched skin during in vivo cell tracking. Though several chemokines were found temporal upregulation induced by mechanical stretch, SDF-1α showed the most significant increase in stretched skin, suggesting a strong connection to migration of MSCs. The in vitro chemotactic assay showed that conditioned medium from mechanically stretched cells induced MSC migration, which could be blocked with the CXCR4 antagonist AMD3100, as effectively as medium containing 50 ng/ml rat recombinant SDF-1α. Results from in vivo study also showed that MSC migration to mechanically stretched skin was significantly blocked by AMD3100. MSC Group had significant advantage in skin regeneration than AMD-MSC Group. Moreover, migrating MSCs expressed differentiation markers, suggesting a contribution of MSCs to skin regeneration through differentiation. When in vivo blocking of SDF-1α/CXCR4 pathway, transplanted MSC effect in promoting skin regeneration was sharply decreased.
Figure 1 [Fig. 1], Figure 2 [Fig. 2]
Conclusions: Mechanical stretching can upregulate SDF-1α in skin and recruit circulating MSCs through the SDF-1α/CXCR4 pathway. Migrated MSCs can promote skin regeneration by differentiating into structural cells and accelerating skin cell proliferation. The results also indicated that MSCs recruited by SDF-1α/CXCR4 pathway had important role in promoting mechanical stretch induced skin regeneration.
