Artikel
Revisiting the go or grow hypothesis in glioma
Proliferation und Migration in Gliomen
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Veröffentlicht: | 26. Juni 2020 |
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Gliederung
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Objective: Glioblastomas continue to be a therapeutic challenge due to their aggressive growth, which is both rapid and diffusely infiltrative. It has been postulated in the past that tumor cells cannot invade ("go") and divide ("grow") at the same moment of time, disconnecting both pathological hallmarks of the disease.
Methods: To investigate the interrelation of glioblastoma cell invasion and proliferation patterns, we established a novel intravital microscopy methodology which allows to track invasion and proliferation in glioma microregions at the same time: by long-term following individual patient-derived glioblastoma cells expressing fluorescent markers which depict both the cytoplasm and the mitotic history of individual tumor cells in different disease stages and tumor regions.
Results: We found that, in contrast to the prevailing hypothesis, the most invasive tumor cells were also the most proliferative when followed over weeks. In established tumor regions, those glioblastoma cells that were particularly well connected to other tumor cells in the tumor microtube-connected tumor network were slow-cycling over weeks, supporting their potential stem-like identity (Figure 1 [Fig. 1]).
Conclusion: In summary, this data challenges the current "go-or-grow" hypothesis in glioma progression, and provides valuable insights into the interrelation of central traits of malignancy in glioma.