Artikel
Lipid profiles of porcine brain tissues obtained by picosecond-infrared laser ablation correlate with anatomical brain regions
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Autoren
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Franz Ricklefs
- Klinik und Poliklinik für Neurochirurgie, Universitätsklinikum Hamburg-Eppendorf, Germany
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Marcus Wurlitzer
- Institut für Klinische Chemie und Laboratoriumsmedizin, Universitätsklinikum Hamburg-Eppendorf, Germany
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Karsten Rinas
- Institut für Klinische Chemie und Laboratoriumsmedizin, Universitätsklinikum Hamburg-Eppendorf, Germany
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Hannes Petersen
- Klinik und Poliklinik für Hals-Nasen-Ohrenheilkunde, Universitätsklinikum Hamburg-Eppendorf, Germany
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Jan Regelsberger
- Klinik und Poliklinik für Neurochirurgie, Universitätsklinikum Hamburg-Eppendorf, Germany
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Hartmut Schlüter
- Institut für Klinische Chemie und Laboratoriumsmedizin, Universitätsklinikum Hamburg-Eppendorf, Germany
| Veröffentlicht: | 8. Juni 2016 |
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Gliederung
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Objective: The differentiation of healthy tissue to cancerous tissue is still crucial in neurosurgery. The aim of this study was to analyze the capability and applicability of mass spectrometric tissue characterization by lipid profiles after picosecond-infrared laser (PIRL) ablation. PIRL has a huge advantage compared to competing techniques, including rapid evaporation ionization mass spectrometry (REIMS), since there is no thermal damage to the tissue, enabling the possibility for an extended resection of tumor tissue without harming the surrounding healthy brain parenchyma. This is the first preclinical study showing the possibility to differentiate brain regions by PIRL ablation and mass spectrometric (MS) lipidomics in a porcine model.
Method: Pig brain tissue samples were obtained immediately after death. Cerebrum and cerebellum were dissected for white matter and cortex and were snap frozen until further analysis. Samples were ablated by cold vaporization using PIRL, a pulsed infrared laser with 400 ps pulse duration and a wavelength of 2.94 µm. The condensates of the PIRL tissue aerosoles were directly infused into a MS in positive and negative ion mode covering a range of m/z 450-1500. Data were analyzed using Xcalibur 3.0 and Mathematica.
Results: We demonstrate the recognition of porcine brain regions by the MS lipid profiles of the condensates of the PIRL tissue aerosoles. A principal component analysis of the signals detected in the mass spectra of various brain regions resulted in a clear separation of the different brain tissues. The remaining tissues did not have any burn marks after laser irradiation.
Conclusions: In this preclinical study we showed that different brain regions could be recognized by their lipid profiles obtained by ablation of the tissues with PIRL and subsequent lipidomics of the condensates of the PIRL aerosoles. Because there is no additional sample preparation procedure required, the presented method in the future should be suitable for transferring the PIRL aerosole directly into a MS, enabling real-time feedback applications. The softness of the laser ablation in combination with real-time tissue recognition have a tremendous translational potential into the neurosurgical setting.
