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67. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie (DGNC)
Joint Meeting mit der Koreanischen Gesellschaft für Neurochirurgie (KNS)

Deutsche Gesellschaft für Neurochirurgie (DGNC) e. V.

12. - 15. Juni 2016, Frankfurt am Main

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VOGiM: A versatile brain tumor angiogenesis interface in neurooncology

Meeting Abstract

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  • Nicolai Savaskan - Department of Neurosurgery, Universitätsklinikum Erlangen, Friedrich-Alexander University Erlangen-Nürnberg, Erlangen, Germany
  • Ali Ghoochani - Department of Neurosurgery, Universitätsklinikum Erlangen, Friedrich-Alexander University Erlangen-Nürnberg, Erlangen, Germany
  • Eduard Yakubov - Department of Neurosurgery, Universitätsklinikum Erlangen, Friedrich-Alexander University Erlangen-Nürnberg, Erlangen, Germany
  • Michael Buchfelder - Department of Neurosurgery, Universitätsklinikum Erlangen, Friedrich-Alexander University Erlangen-Nürnberg, Erlangen, Germany
  • Ilker Y. Eyupoglu - Department of Neurosurgery, Universitätsklinikum Erlangen, Friedrich-Alexander University Erlangen-Nürnberg, Erlangen, Germany

Deutsche Gesellschaft für Neurochirurgie. 67. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie (DGNC), 1. Joint Meeting mit der Koreanischen Gesellschaft für Neurochirurgie (KNS). Frankfurt am Main, 12.-15.06.2016. Düsseldorf: German Medical Science GMS Publishing House; 2016. DocMI.10.04

doi: 10.3205/16dgnc285, urn:nbn:de:0183-16dgnc2852

Veröffentlicht: 8. Juni 2016

© 2016 Savaskan et al.
Dieser Artikel ist ein Open-Access-Artikel und steht unter den Lizenzbedingungen der Creative Commons Attribution 4.0 License (Namensnennung). Lizenz-Angaben siehe http://creativecommons.org/licenses/by/4.0/.

Gliederung

Text

Objective: In the past, a variety of assays have been established to study angiogenesis, but very few allow investigations in a model with organotypic cellular and microenvironmental composition. Previously, we established an Organotypic Glioma Invasion Model (OGIM) which allows monitoring in real-time tumor invasion, metastasis and angiogenesis in an in-vitro experiment with in-vivo conditions.

Method: Therefore, we extended this ex-vivo system by tracking the distribution of genetically marked glioma cells (RFP, GFP) and vascular components.

Results: In this model, we found that the blood-vessel architecture is altered drastically in and around the tumor-bulk in comparison to normal brain tissue without tumor contact. Within the tumor, the blood vessels show ranging diameters with erratic alterations of vessel types and course. The peritumoral region is characterized by the absence of large diameter vessels, or metarterioles whereas unaffected brain regions display an even and regular distribution of vessels from capillaries, arterioles and metarterioles. We classified these alterations in physiological vessel architecture with large diameter vessels at the pial surface and smaller vessels radiating into the cortex. In contrast, vessels in the peritumoral area show a heterogeneous and diffuse architecture. Vessels in the tumor core range from „bigger than normal“ to „capillary size“ diameter and represent an altered architecture in terms of variations, diameter and distribution.

Conclusions: Thus, our novel VOGiM model system represents a versatile system to study tumor – brain – blood vessel interaction and thus represents a bridging assay for purely cell based in-vitro assays and in-vivo animal experiments.