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67. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie (DGNC)
Joint Meeting mit der Koreanischen Gesellschaft für Neurochirurgie (KNS)

Deutsche Gesellschaft für Neurochirurgie (DGNC) e. V.

12. - 15. Juni 2016, Frankfurt am Main

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Soft alginate hydrogel attenuates scar formation and promotes functional recovery after spinal cord injury in rats

Meeting Abstract

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  • Kerim H. Sitoci-Ficici - Klinik für Neurochirurgie, Universitätsklinikum Carl Gustav Carus, TU Dresden, Dresden, Germany
  • Robert Later - Klinik für Neurochirurgie, Universitätsklinikum Carl Gustav Carus, TU Dresden, Dresden, Germany
  • Ortrud Uckermann - Klinik für Neurochirurgie, Universitätsklinikum Carl Gustav Carus, TU Dresden, Dresden, Germany
  • Roberta Galli - Clinical Sensoring and Monitoring, Klinik für Anesthesiologie und Intensivmedizin, Medizinische Fakultät, TU Dresden, Dresden, Germany
  • Sandra Tamosaiyte - Clinical Sensoring and Monitoring, Klinik für Anesthesiologie und Intensivmedizin, Medizinische Fakultät, TU Dresden, Dresden, Germany; Faculty of Physics, Department of General Physics and Spectroscopy, Vilnius University, Vilnius, Lithuania
  • Elke Leipnitz - Klinik für Neurochirurgie, Universitätsklinikum Carl Gustav Carus, TU Dresden, Dresden, Germany
  • Rudolf Beiermeister - Zentrum für Translationale Knochen- Gelenk- und Weichteilforschung, TU Dresden, Dresden, Germany
  • Michael Gelinksy - Zentrum für Translationale Knochen- Gelenk- und Weichteilforschung, TU Dresden, Dresden, Germany; CRTD / DFG-Zentrum für Regenerative Behandlung Dresden – Cluster of Excellence, TU Dresden, Dresden, Germany
  • Gabriele Schackert - Klinik für Neurochirurgie, Universitätsklinikum Carl Gustav Carus, TU Dresden, Dresden, Germany
  • Matthias Kirsch - Klinik für Neurochirurgie, Universitätsklinikum Carl Gustav Carus, TU Dresden, Dresden, Germany; CRTD / DFG-Zentrum für Regenerative Behandlung Dresden – Cluster of Excellence, TU Dresden, Dresden, Germany

Deutsche Gesellschaft für Neurochirurgie. 67. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie (DGNC), 1. Joint Meeting mit der Koreanischen Gesellschaft für Neurochirurgie (KNS). Frankfurt am Main, 12.-15.06.2016. Düsseldorf: German Medical Science GMS Publishing House; 2016. DocDI.04.09

doi: 10.3205/16dgnc118, urn:nbn:de:0183-16dgnc1182

Veröffentlicht: 8. Juni 2016

© 2016 Sitoci-Ficici et al.
Dieser Artikel ist ein Open-Access-Artikel und steht unter den Lizenzbedingungen der Creative Commons Attribution 4.0 License (Namensnennung). Lizenz-Angaben siehe http://creativecommons.org/licenses/by/4.0/.

Gliederung

Text

Objective: The formation of a scar following traumatic spinal cord injury (SCI) is a great hurdle for regenerative processes. This renders the injury site non-permissive for potential sprouting axons. Recently, we demonstrated that soft alginate hydrogel supported neurite outgrowth and protected neurons against oxidative stress in vitro. The purpose of the current study was to investigate the effects of such soft alginate hydrogels on locomotor recovery in a hemimyelonectomy model of spinal cord injury in rats.

Method: A rat SCI model consisted of a left-sided 2 mm (2X2x1,5 mm gap) hemimyelonectomy at the thoracic vertebral level 9. 21 rats were treated with alginate and 21 rats were in the control group. 7 rats from each group were sacrificed for morphochemical studies via Fourier-transform infrared (FT-IR) spectroscopy at 1 month post injury. In the rest (n 28), the functional recovery was assessed via BBB score until 5 mo. after injury. At 5 mo. postinjury DiI was implanted in 7 alginate and 7 control rats for anterograde in vivo axonal tracing and 1 month thereafter these rats were sacrificed. At 6 mo. post injury the remaining 14 rats (7 alginate, 7 control) were again examined by FT-IR spectroscopy.

Results: Over the observation period, 2 mm alginate rats demonstrated significantly improved functional recovery compared to controls (general linear model with right hindlimb p=0.023 F 3.253; left hindlimb p<0.001 F 5.559). Anterograde in vivo tracing of axons revealed similar distribution of DiI-axons in the control and alginate groups; DiI-labelling was abrogated at the wound margin, but was continuous on the lesion-free side of the spinal cord. FT-IR spectroscopy revealed that alginate was still in situ in the 6 mo. postinjury. We observed a higher lipid content (meyelin) in the samples with alginate (two-tailed t-test, p = 0.019, p = 0.0095, p = 0.0095 for 1735 cm-1, 1466 cm-1 and 1225 cm-1 bands, respectively) and a lower content of collagenous tissue (fibrous scar) in the samples with alginate implant six months after injury (t-test, p = 0.0153).

Conclusions: Implantation of soft alginate hydrogel improved functional recovery in rats after SCI and inhibited demyelination and fibrous scarring. FT-IR spectroscopy depicts label-free the tissue alteration following SCI. It offers a great tool for combining chemical and morphological tissue imaging at the molecular level.