gms | German Medical Science

64. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie (DGNC)

Deutsche Gesellschaft für Neurochirurgie (DGNC) e. V.

26. - 29. Mai 2013, Düsseldorf

Behavioral and electrophysiological validation of rotenone induced rat model of Parkinson's disease

Meeting Abstract

  • Christof von Wrangel - Klinik für Neurochirurgie, Medizinischen Hochschule Hannover
  • Kerstin Schwabe - Klinik für Neurochirurgie, Medizinischen Hochschule Hannover
  • Joachim K. Krauss - Klinik für Neurochirurgie, Medizinischen Hochschule Hannover
  • Mesbah Alam - Klinik für Neurochirurgie, Medizinischen Hochschule Hannover

Deutsche Gesellschaft für Neurochirurgie. 64. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie (DGNC). Düsseldorf, 26.-29.05.2013. Düsseldorf: German Medical Science GMS Publishing House; 2013. DocMI.04.11

doi: 10.3205/13dgnc311, urn:nbn:de:0183-13dgnc3118

Veröffentlicht: 21. Mai 2013

© 2013 von Wrangel et al.
Dieser Artikel ist ein Open Access-Artikel und steht unter den Creative Commons Lizenzbedingungen ( Er darf vervielfältigt, verbreitet und öffentlich zugänglich gemacht werden, vorausgesetzt dass Autor und Quelle genannt werden.



Objective: The 6-hydroxydopamine (6-OHDA) Parkinson (PD) rat model is based on specific dopamine depletion in the nigrostriatal pathway, which subsequently leads to hyperactivity of the subthalamic nucleus (STN). Chronic rotenone injections similarly lead to loss of dopaminergic neurons in the substantia nigra but in addition also to loss of cholinergic neurons in the pedunculopontine nucleus (PPN), which has been thought to underly certain components of parkinsonian gait. We here evaluated the motor disability and the neuronal firing activity of the STN in the rotenone rat model of PD.

Method: Male Sprague Dawley rats were treated with chronic rotenone injections (2.5 mg / kg bodyweight, i.p.) for 60 days. Control rats received vehicle injections. After the end of the treatment motor coordination was assessed by using the Rotarod test. Thereafter, single unit activities and local field potentials were recorded in the STN during anesthesia with urethane 1.2 g/kg.

Results: Rotenone injected rats spent significantly less time on the Rotarod as compared to vehicle treated rats (p < 0.05). Further, electrophysiological data showed a higher firing rate and higher beta oscillatory activity in the STN (p < 0.05).

Conclusions: Similar as in 6-OHDA injection we found enhanced STN neuronal firing rates as well as increased beta oscillatory activity, key features of PD, in this model. The rotenone-induced rat model of PD should deserve further attention since it covers more aspects than just the dopamine depletion.