Artikel
Molecular neuropathology of epilepsy-associated glioneuronal tumors: functional genome analysis in gangliogliomas
Molekulare Neuropathologie Epilepsie-assoziierter glioneuronaler Tumore: funktionelle Genomanalyse in Gangliogliomen
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Autoren
Veröffentlicht: | 4. Mai 2005 |
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Gliederung
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Objective
The comprehensive analysis of disease-related gene transcripts gains increasing importance for understanding the molecular basis of human diseases. Gangliogliomas constitute the most frequent glioneuronal tumor entity occurring in patients with pharmacoresistant epilepsy. We here report first results of a microarray-based expression profile analysis in gangliogliomas.
Methods
Microarray analysis (Affymetrix U133A) was carried out comparing expression patterns in ganglioglioma vs. adjacent normal control tissue (n= 6 patients). Real time RT-PCR analysis was performed to validate differential gene expression patterns.
Results
A total number of 95 genes was found to be differentially expressed in gangliogliomas vs. controls. Stratification by gene ontology analysis revealed several differentially expressed genes related to neuronal differentiation and migration. In particular, protein kinase C, beta 1 type as well as its binding partner NELL2 are significantly reduced in expression in gangliogliomas.
Conclusions
Gene chip technologies open new avenues for the identification of candidate molecules and mechanisms involved in the pathogenesis of gangliogliomas. Current experiments focus on functional analyses of candidate genes using siRNA approaches.