Artikel
Spectral domain optical coherence tomography: a pilot study for a novel guidance technique for neurosurgical resection of brain tumours
Spektralradar basierte optische Kohärenz-Tomographie: eine Pilotstudie für eine neuartige, assistierende Technik zur neurochirurgischen Resektion von Hirntumoren
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Autoren
Veröffentlicht: | 4. Mai 2005 |
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Gliederung
Text
Objective
Spectral domain optical coherence tomography (SOCT) is a non-invasive imaging technique for tissue structures with a resolution accurate to micrometers. Optical coherence tomography can operate with no tissue contact, and similar to ultrasound B-mode imaging, can generate images based on the reflection of infrared laser light using the inherent optical tissue contrast. We have evaluated this technology for analysis of central nervous system tissues as a potential adjunct to the detection of residual tumour during resection of brain tumours.
Methods
We have used a near infrared 830nm laser to analyse an orthotopic mouse glioma model of invasive and non-invasive human glioma cell lines and native or formalin fixed biopsy specimens of normal brain and glioma tissue of different malignancy grades to correlate the optical tissue analysis with histological parameters. The SOCT laser unit was coupled to a neuronavigation system and was used in a non-contact technique to image central nervous system tissue guided by the signal intensity of tissue structures on magnetic resonance imaging in vivo.
Results
OCT imaging of normal cortical tissues allowed visualisation of arachnoid and pial layers and cortical microvessels at a resolution of <10 μm. Cortex or white matter could be delineated from solid tumour tissue and invaded adjacent brain both by appearance on SOCT and by an automated analysis of the inherent optical tissue contrast. Integration of SOCT into the neuronavigation allowed a directed analysis of tissue structures and a correlation with signal abnormalities on MRI.
Conclusions
Spectral domain optical coherence tomography allows non-contact / non-invasive analysis of central nervous system tissues with a penetration depth of 2-3 mm reaching a spatial resolution of approximately 4-15 μm. This technique allows detection of the distortion of the normal tissue architecture by tumour. The analysis of the optical tissue characteristics enables a quantitative analysis of a backscatter coefficient differentiating normal tissue, invading tumour cells, or solid tumour.