Artikel
The effect of Mitomycin-C preventing the epidural fibrosis after lumbar laminectomy in rats
Reduktion der epiduralen Fibrose und Verhinderung der duralen Adhäsion durch Mitomycin-C in einem Laminektomie-Modell der Ratten
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Autoren
Veröffentlicht: | 4. Mai 2005 |
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Gliederung
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Objective
Extensive epidural fibrosis after lumbar spine surgery may be the underlying cause in most cases of failed back surgery syndroms. A large variety of materials has been used to prevent epidural fibrosis with varying results. Mitomycin-C (MMC), an alkylating antibiotic isolated from Streptomyces caespitosus, potentially suppresses fibroblast proliferation after surgical intervention. Based on the data from the previous study, we investigated the effect of MMC in concentration of 0.1 mg/ml reducing the formation of peridural fibrosis and preventing dural adhesions in a rat laminectomy model.
Methods
Eighty adult Wistar rats underwent a lumbar laminectomy. In 40 rats, MMC in concentration of 0.1 mg/ml was locally applied to the laminectomy sites. No treatment was performed in another 40 rats, and served as a control. In 7 rats, the concentration of MMC in cerebrospinal fluid and serum was determined 12 hours postoperatively. 10 rats underwent magnetic resonance imaging 6 weeks postoperatively. 12 weeks after laminectomy, the animals were sacrificed for histological evaluations.
Results
In all MMC-treated laminectomy sites, epidural scarring was significantly reduced and dural adhesions were absent in comparison with control sites. Remarkably, there was an absence of dural adhesions in the majority of laminectomy defects treated with MMC. All control sites showed dense epidural fibrosis with marked dural adherence.
Conclusions
In this experimental model, MMC locally applied with a concentration of 0.1 mg/ml effectively reduces epidural fibrosis avoiding dural adherence without any side effects.