gms | German Medical Science

76th Annual Meeting of the German Society of Oto-Rhino-Laryngology, Head and Neck Surgery

German Society of Oto-Rhino-Laryngology, Head and Neck Surgery

04.05. - 08.05.2005, Erfurt

Article

Send article

Differential proteome analysis of tonsil material from children with recurrent acute or chronic tonsillitis in comparison with hyperplasia in order to identify diagnostic marker proteins

Meeting Abstract

Search Medline for

  • corresponding author Tino Just - Department of Otorhinolaryngology, Head and Neck Surgery, University of Rostock, Germany
  • Evariste Gafumbegete - Institute for Pathology, University of Rostock
  • Jan Gramberg - Proteome Center Rostock, University of Rostock
  • Ines Prüfer - Proteome Center Rostock, University of Rostock
  • Bruno Ringel - Proteome Center Rostock, University of Rostock
  • Stefan Mikkat - Proteome Center Rostock, University of Rostock
  • author Hans Wilhelm Pau - Department of Otorhinolaryngology, Head and Neck Surgery, University of Rostock, Germany
  • Michael O. Glocker - Proteome Center Rostock, University of Rostock

Deutsche Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie. 76. Jahresversammlung der Deutschen Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie e.V.. Erfurt, 04.-08.05.2005. Düsseldorf, Köln: German Medical Science; 2005. Doc05hno055

The electronic version of this article is the complete one and can be found online at: http://www.egms.de/en/meetings/hno2005/05hno040.shtml

Published: September 22, 2005

© 2005 Just et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.en). You are free: to Share – to copy, distribute and transmit the work, provided the original author and source are credited.

Outline

Text

Differential proteome analysis was used to compare protein pattern from fresh-frozen tonsils derived from children with recurrent acute or chronic tonsillitis, and tonsil hyperplasia, respectively. In order to find disease associated marker proteins, protein pattern of both entities were compared.

Palatine tonsils from 37 children, aged between 2 and 11, were removed surgically. Thirty-seven extracts from those children were analyzed (23 from recurrent acute tonsillitis and 14 from hyperplasia). Two-dimensional gel electrophoresis (2-DE) of the soluble proteins was performed from hyperplasia (n = 2) and recurrent tonsillitis (n = 5) in triplicate. Differential computer-based 2-DE-gel analysis was carried out using two 2D reference gels of each entity. Selected protein spots were excised from the gels, digested with trypsin and identified by peptide mass fingerprinting with MALDI-TOF MS.

600 Protein spots were visualized routinely on each gel using Coomassie staining. A strong similarity in abundance of protein spots within each group was apparent. Only those differences that persisted in the rigorous differential analysis were addressed as differentially expressed, resulting in rather small differences in protein patterns between tonsillitis and hyperplasia. Clear abundance differences between the two groups were found for heat shock protein 27 (HSP27) and UMP-CMP kinase. The spots of both proteins were more intensive in recurrent tonsillitis. HSP27 ELISA analyses were performed to verify the different abundance of HSP27 with extracts from 32 frozen juvenile tonsils. Higher HSP27 level were found in children with tonsillitis in comparison to children with hyperplasia. In further investigations we will evaluate the applicability of HSP27 as diagnostic marker for tonsil disease.