gms | German Medical Science

102. Jahrestagung der DOG

Deutsche Ophthalmologische Gesellschaft e. V.

23. bis 26.09.2004, Berlin

Measurement of Triamcinolone-acetonide concentration after filtering of preservative

Meeting Abstract

  • C. Papoulis - Augenklinik, Evangelisches Stift, Koblenz
  • corresponding author U.H.M. Spandau - Augenklinik, Evangelisches Stift, Koblenz
  • M. Derse - Augenklinik, Evangelisches Stift, Koblenz
  • P. Schmitz-Valckenberg - Augenklinik, Evangelisches Stift, Koblenz
  • J.B. Jonas - Augenklinik, Universität Mannheim

Evidenzbasierte Medizin - Anspruch und Wirklichkeit. 102. Jahrestagung der Deutschen Ophthalmologischen Gesellschaft. Berlin, 23.-26.09.2004. Düsseldorf, Köln: German Medical Science; 2004. Doc04dogP 183

The electronic version of this article is the complete one and can be found online at:

Published: September 22, 2004

© 2004 Papoulis et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( You are free: to Share – to copy, distribute and transmit the work, provided the original author and source are credited.




In clinical studies different dosages of triamcinolone acetonide are used: 4mg unfiltered triamcinolone acetonide and 25mg filtered triamcinolone acetonide (without benzylalcohol). Purpose of this study was to determine the concentration of triamcinolone acetonide probes with and without filtering.


A hospital pharmacy produced 5 not filtered 4mg and 5 filtered 25mg probes. As control five not filtered 25mg triamcinolone acetonide probes were produced. The probes were sent to a reference laboratory. Using a High Phase Liquid Cromatography (HPLC) Assay the soluble triamcinolone acetonide as well the crystals were measured.


The HPLC assay measured a triamcinolone acetonide concentration of 3.1±0.6mg (± SD) from the 4mg probes and 12.8±0.7mg (± SD) from the 25mg probes. The five not filtered 25mg syringes used as control showed a concentration of 23.4±2.3mg (± SD).


Depending on the employed method filtering of triamcinolone acetonide results in significant reduction of end-concentration. These results should be considered when interpreting clinical studies with triamcinolone acetonide.