Article
Measurement of Triamcinolone-acetonide concentration after filtering of preservative
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Authors
Published: | September 22, 2004 |
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Outline
Text
Objective
In clinical studies different dosages of triamcinolone acetonide are used: 4mg unfiltered triamcinolone acetonide and 25mg filtered triamcinolone acetonide (without benzylalcohol). Purpose of this study was to determine the concentration of triamcinolone acetonide probes with and without filtering.
Methods
A hospital pharmacy produced 5 not filtered 4mg and 5 filtered 25mg probes. As control five not filtered 25mg triamcinolone acetonide probes were produced. The probes were sent to a reference laboratory. Using a High Phase Liquid Cromatography (HPLC) Assay the soluble triamcinolone acetonide as well the crystals were measured.
Results
The HPLC assay measured a triamcinolone acetonide concentration of 3.1±0.6mg (± SD) from the 4mg probes and 12.8±0.7mg (± SD) from the 25mg probes. The five not filtered 25mg syringes used as control showed a concentration of 23.4±2.3mg (± SD).
Conclusions
Depending on the employed method filtering of triamcinolone acetonide results in significant reduction of end-concentration. These results should be considered when interpreting clinical studies with triamcinolone acetonide.