gms | German Medical Science

27th German Cancer Congress Berlin 2006

German Cancer Society (Frankfurt/M.)

22. - 26.03.2006, Berlin

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Orthotopic tumor transplantation as a model for induction of minimal residual disease in nude mice

Meeting Abstract

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  • corresponding author presenting/speaker Bertram Illert - Chirurgische Klinik I, Universitätsklinik Würzburg, Deutschland
  • Dominik Stehle - Chirurgische Klinik I, Universitätsklinik Würzburg
  • Christoph Otto - Abteilung Experimentelle Chirurgie der Chirurgischen Universitätsklinik Würzburg

27. Deutscher Krebskongress. Berlin, 22.-26.03.2006. Düsseldorf, Köln: German Medical Science; 2006. DocPO408

The electronic version of this article is the complete one and can be found online at: http://www.egms.de/en/meetings/dkk2006/06dkk518.shtml

Published: March 20, 2006

© 2006 Illert et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.en). You are free: to Share – to copy, distribute and transmit the work, provided the original author and source are credited.

Outline

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Objective: Detection of disseminated tumor cells in blood and bone marrow is an indicator for minimal residual disease (MRD). To study adjuvant therapies and their effectiveness in different gastrointestinal cancers, animal models for MRD would be suitable.

Methods: Tumor specimen from gastric (n=20), pancreatic (n=20) or colon cancer (n=10) were transplanted in nude mice. Human derived tumors were transplantated orthotopically due to their site of origin. Tumor growth, metastases and induction of MRD were compared in all groups. Animals were sacrificed after 4-14 weeks and a nested RT-PCR for human CK20 from bone marrow and blood of mice was performed for detection of disseminated tumor cells.

Results: Tumor growth was varying in dependence from the organ specimen between 5% (colon) and 100% (gastric) in the different groups. Development of distant metastases (lymph nodes, liver, lung) and MRD was observed in animals with gastric cancer. Pancreatic tumors showed an aggressive behavior with fast local and destructive growth, while animals after transplantation of colon cancer specimen developed tumors only occasionally and developed no distant metastases.

Conclusions: The model of orthotopic transplantation of tumor specimen for dissemination of tumor cells in bone marrow and blood was provable for gastric cancer and is a usefull model for the examination of therapies against MRD. The model is less suitable for pancreatic cancer due to inconstant results and it is not suitable for induction of metastasizing course in colon cancer.