gms | German Medical Science

27. Deutscher Krebskongress

Deutsche Krebsgesellschaft e. V.

22. - 26.03.2006, Berlin

Orthotopic tumor transplantation as a model for induction of minimal residual disease in nude mice

Meeting Abstract

Suche in Medline nach

  • corresponding author presenting/speaker Bertram Illert - Chirurgische Klinik I, Universitätsklinik Würzburg, Deutschland
  • Dominik Stehle - Chirurgische Klinik I, Universitätsklinik Würzburg
  • Christoph Otto - Abteilung Experimentelle Chirurgie der Chirurgischen Universitätsklinik Würzburg

27. Deutscher Krebskongress. Berlin, 22.-26.03.2006. Düsseldorf, Köln: German Medical Science; 2006. DocPO408

Die elektronische Version dieses Artikels ist vollständig und ist verfügbar unter: http://www.egms.de/de/meetings/dkk2006/06dkk518.shtml

Veröffentlicht: 20. März 2006

© 2006 Illert et al.
Dieser Artikel ist ein Open Access-Artikel und steht unter den Creative Commons Lizenzbedingungen (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.de). Er darf vervielf&aauml;ltigt, verbreitet und &oauml;ffentlich zug&aauml;nglich gemacht werden, vorausgesetzt dass Autor und Quelle genannt werden.


Gliederung

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Objective: Detection of disseminated tumor cells in blood and bone marrow is an indicator for minimal residual disease (MRD). To study adjuvant therapies and their effectiveness in different gastrointestinal cancers, animal models for MRD would be suitable.

Methods: Tumor specimen from gastric (n=20), pancreatic (n=20) or colon cancer (n=10) were transplanted in nude mice. Human derived tumors were transplantated orthotopically due to their site of origin. Tumor growth, metastases and induction of MRD were compared in all groups. Animals were sacrificed after 4-14 weeks and a nested RT-PCR for human CK20 from bone marrow and blood of mice was performed for detection of disseminated tumor cells.

Results: Tumor growth was varying in dependence from the organ specimen between 5% (colon) and 100% (gastric) in the different groups. Development of distant metastases (lymph nodes, liver, lung) and MRD was observed in animals with gastric cancer. Pancreatic tumors showed an aggressive behavior with fast local and destructive growth, while animals after transplantation of colon cancer specimen developed tumors only occasionally and developed no distant metastases.

Conclusions: The model of orthotopic transplantation of tumor specimen for dissemination of tumor cells in bone marrow and blood was provable for gastric cancer and is a usefull model for the examination of therapies against MRD. The model is less suitable for pancreatic cancer due to inconstant results and it is not suitable for induction of metastasizing course in colon cancer.