gms | German Medical Science

43. Jahrestagung der Deutschen Gesellschaft der Plastischen, Rekonstruktiven und Ästhetischen Chirurgen e. V. (DGPRÄC), 17. Jahrestagung der Vereinigung der Deutschen Ästhetisch-Plastischen Chirurgen e. V. (VDÄPC)

13.09. - 15.09.2012, Bremen

The influence of extra-terrestrial dust stimulants on human cutaneous wound healing in ex vivo model

Meeting Abstract

  • presenting/speaker G. Valiyeva - Medizinische Hochschule Hannover, Plastische Wiederherstellungs und Hand Chirurgie, Hannover, Germany
  • R. Schnabel - Medizinische Hochschule Hannover, Plastische Wiederherstellungs und Hand Chirurgie, Hannover, Germany
  • A. Jokuszies - Medizinische Hochschule Hannover, Plastische Wiederherstellungs und Hand Chirurgie, Hannover, Germany
  • P.M. Vogt - Medizinische Hochschule Hannover, Plastische Wiederherstellungs und Hand Chirurgie, Hannover, Germany
  • M. Duenne - Medizinische Hochschule Hannover, Plastische Wiederherstellungs und Hand Chirurgie, Hannover, Germany
  • K. Brix - Medizinische Hochschule Hannover, Plastische Wiederherstellungs und Hand Chirurgie, Hannover, Germany
  • U. Mirastschijski - Medizinische Hochschule Hannover, Plastische Wiederherstellungs und Hand Chirurgie, Hannover, Germany

Deutsche Gesellschaft der Plastischen, Rekonstruktiven und ─sthetischen Chirurgen. Vereinigung der Deutschen ─sthetisch-Plastischen Chirurgen. 43. Jahrestagung der Deutschen Gesellschaft der Plastischen, Rekonstruktiven und ─sthetischen Chirurgen (DGPR─C), 17. Jahrestagung der Vereinigung der Deutschen ─sthetisch-Plastischen Chirurgen (VD─PC). Bremen, 13.-15.09.2012. DŘsseldorf: German Medical Science GMS Publishing House; 2012. DocFTIP04

doi: 10.3205/12dgpraec133, urn:nbn:de:0183-12dgpraec1336

Published: September 10, 2012

© 2012 Valiyeva et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.en). You are free: to Share – to copy, distribute and transmit the work, provided the original author and source are credited.


Outline

Text

In 2006 NASA published about its plans to build a manned lunar station in order to undertake missions to Mars in the future. In the course of it research projects have been conducted which examine the influence of lunar and martian dust on human health. The present study investigates the effect of lunar and martian dust simulants (LDS, MDS) in comparison with earth dust (ED) on viability, proliferation and migration as well as local inflammatory reactions during wound closure in an ex vivo human skin wound model. Here, skin explants, punched as 6 mm full-thickness skin with a central 3 mm epidermal wound were cultured with LDS or MDS over 4 and 8 days in comparison with ED and no dust. The cultured tissue and conditioned medium were submitted to histological, immunohistochemical (Ki67, Caspase-3) and biochemical analyses (hydroxyproline assay, gelatin zymography, IL-6 and TNF-α ELISA). There were no significant changes regarding wound closure, proliferation, apoptosis and tissue degradation between groups. However, as to the control group without dust, a significant higher level of the proinflammatory cytokine IL-6 and reduced MMP-2 were observed on day 8. Thus, contamination with extra-terrestrial dust may lead to disturbances in skin wound healing through enhanced inflammatory reaction triggered by increased IL-6. Moreover, significantly reduced active MMP-2, responsible for dermal remodeling, may lead to delayed tissue regeneration and scar formation. As it is advocated for wounds contaminated with earth dust, an extensive surgical wound debridement should also be performed in case of contact with extraterrestrian dust to ensure unimpaired wound healing.